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Anti-Phospho-PKC δ (Tyr311) Rabbit Antibody [P4C22]

Catalog No.: F3958

Application: Reactivity: Human

Usage Information

Dilution
WB1:2500 - 1:5000 IP1:40 IHC1:100

Application
WB, IP, IHC

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
77 kDa 78 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-Phospho-PKC δ (Tyr311) Rabbit Antibody [P4C22] detects endogenous levels of total PKC δ protein only when phosphorylated at Tyr 311.

Clone
P4C22

Synonym(s)
PKCD, PRKCD, Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta

Background
Phosphorylation of Protein Kinase C delta (PKCδ) at tyrosine 311 (Tyr311) is a key regulatory modification located in the hinge region between its regulatory and catalytic domains. This phosphorylation, mediated by Src family kinases upon cellular stimuli such as oxidative stress or receptor activation, enhances PKCδ’s kinase activity and promotes its translocation to different cellular compartments, including the nucleus. Tyr311 phosphorylation is crucial for PKCδ’s role in platelet activation and thromboxane production, thereby influencing blood clotting and thrombosis. It also regulates apoptotic signaling by facilitating PKCδ cleavage and nuclear localization. Mutation at Tyr311 significantly reduces PKCδ activity and impairs these downstream cellular processes. This site-specific phosphorylation integrates redox signaling with kinase regulation, leading to inflammation, cancer, and cardiovascular disorders.

Background

Phosphorylation of Protein Kinase C delta (PKCδ) at tyrosine 311 (Tyr311) is a key regulatory modification located in the hinge region between its regulatory and catalytic domains. This phosphorylation, mediated by Src family kinases upon cellular stimuli such as oxidative stress or receptor activation, enhances PKCδ’s kinase activity and promotes its translocation to different cellular compartments, including the nucleus. Tyr311 phosphorylation is crucial for PKCδ’s role in platelet activation and thromboxane production, thereby influencing blood clotting and thrombosis. It also regulates apoptotic signaling by facilitating PKCδ cleavage and nuclear localization. Mutation at Tyr311 significantly reduces PKCδ activity and impairs these downstream cellular processes. This site-specific phosphorylation integrates redox signaling with kinase regulation, leading to inflammation, cancer, and cardiovascular disorders.

References
https://pubmed.ncbi.nlm.nih.gov/33932405/ https://pubmed.ncbi.nlm.nih.gov/11381116/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%