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Anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19) Rabbit Antibody [C13P19]

Catalog No.: F1242

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IF1:400 - 1:800

Application
WB, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
18 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19) Rabbit Antibody [C13P19] detects endogenous levels of myosin light chain 2 protein only when phosphorylated at Thr18 and Ser19.

Clone
C13P19

Synonym(s)
Myosin regulatory light polypeptide 9, 20 kDa myosin light chain (LC20), MLC-2C, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, MYL9, MLC2, MRLC1, MYRL2

Background
Phospho-Myosin Light Chain 2 (Thr18/Ser19) (MLC2) refers to the regulatory light chain of myosin II, which is phosphorylated at threonine 18 (Thr18) and serine 19 (Ser19) residues by myosin light chain kinase (MLCK) and other kinases. Structurally, MLC2 is composed of a single polypeptide associated with the myosin heavy chain, and its N-terminal region contains the key phosphorylation sites that regulate myosin motor activity. MLC2 is widely expressed in smooth muscle, nonmuscle cells, and platelets, where it plays a central role in cytoskeletal dynamics, contractility, and motile processes. Phosphorylation at Ser19, and to a lesser extent Thr18, activates myosin II by enhancing its ATPase activity and promoting filament assembly, leading to actin-myosin interactions essential for cell contraction, migration, and shape changes. Distinct signaling pathways differentially regulate these phosphorylation sites: Ser19 is rapidly phosphorylated downstream of Ca²⁺/MLCK-dependent pathways, driving immediate contractile responses, whereas Thr18 phosphorylation is slower and often linked to Rho kinase–dependent pathways, fine-tuning contractility. Together, Thr18/Ser19 phosphorylation provides a graded mechanism to control myosin II activity, enabling precise regulation of actomyosin-mediated processes such as platelet shape change, stress fiber formation, and cellular motility.

Background

Phospho-Myosin Light Chain 2 (Thr18/Ser19) (MLC2) refers to the regulatory light chain of myosin II, which is phosphorylated at threonine 18 (Thr18) and serine 19 (Ser19) residues by myosin light chain kinase (MLCK) and other kinases. Structurally, MLC2 is composed of a single polypeptide associated with the myosin heavy chain, and its N-terminal region contains the key phosphorylation sites that regulate myosin motor activity. MLC2 is widely expressed in smooth muscle, nonmuscle cells, and platelets, where it plays a central role in cytoskeletal dynamics, contractility, and motile processes. Phosphorylation at Ser19, and to a lesser extent Thr18, activates myosin II by enhancing its ATPase activity and promoting filament assembly, leading to actin-myosin interactions essential for cell contraction, migration, and shape changes. Distinct signaling pathways differentially regulate these phosphorylation sites: Ser19 is rapidly phosphorylated downstream of Ca²⁺/MLCK-dependent pathways, driving immediate contractile responses, whereas Thr18 phosphorylation is slower and often linked to Rho kinase–dependent pathways, fine-tuning contractility. Together, Thr18/Ser19 phosphorylation provides a graded mechanism to control myosin II activity, enabling precise regulation of actomyosin-mediated processes such as platelet shape change, stress fiber formation, and cellular motility.

References
https://pubmed.ncbi.nlm.nih.gov/20670370/ https://pubmed.ncbi.nlm.nih.gov/17928407/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%