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Anti-PERK Rabbit Antibody [A22L11]

Catalog No.: F4128

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IP1:50 IHC1:1000 - 1:4000

Application
WB, IP, IHC

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
140 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-PERK Rabbit Antibody [A22L11] detects endogenous levels of total PERK protein.

Clone
A22L11

Synonym(s)
Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, Protein tyrosine kinase EIF2AK3, EIF2AK3, PEK

Background
PERK is a key ER-resident transmembrane kinase that acts as a major sensor and transducer of endoplasmic reticulum (ER) stress, playing a vital role in cellular homeostasis and fate determination. PERK contains an ER luminal domain that detects accumulation of misfolded proteins, and a cytoplasmic kinase domain that gets activated by homodimerization and autophosphorylation upon ER stress. Activated PERK phosphorylates the eukaryotic initiation factor 2 alpha (eIF2α) at Ser51, attenuating global protein synthesis to reduce the load of new proteins entering the ER while selectively upregulating stress response genes via transcription factors such as ATF4. This regulatory mechanism balances cell survival through autophagy or triggers apoptosis under prolonged stress. PERK activity is intricately regulated by its association with ER chaperones such as GRP78 (BiP), as well as by phosphorylation of critical residues within its kinase domain. PERK is present at mitochondria-associated membranes (MAMs), where it mediates ER-mitochondrial crosstalk that is crucial for oxidative stress sensing and apoptotic signaling. Pharmacological inhibition of PERK reduces pathology in neurodegenerative disease models but can cause side effects, indicating the importance of precise regulation. Among ER stress sensors, PERK modulates cell fate decisions, acting as a potential therapeutic target for diseases characterized by ER stress and protein misfolding.

Background

PERK is a key ER-resident transmembrane kinase that acts as a major sensor and transducer of endoplasmic reticulum (ER) stress, playing a vital role in cellular homeostasis and fate determination. PERK contains an ER luminal domain that detects accumulation of misfolded proteins, and a cytoplasmic kinase domain that gets activated by homodimerization and autophosphorylation upon ER stress. Activated PERK phosphorylates the eukaryotic initiation factor 2 alpha (eIF2α) at Ser51, attenuating global protein synthesis to reduce the load of new proteins entering the ER while selectively upregulating stress response genes via transcription factors such as ATF4. This regulatory mechanism balances cell survival through autophagy or triggers apoptosis under prolonged stress. PERK activity is intricately regulated by its association with ER chaperones such as GRP78 (BiP), as well as by phosphorylation of critical residues within its kinase domain. PERK is present at mitochondria-associated membranes (MAMs), where it mediates ER-mitochondrial crosstalk that is crucial for oxidative stress sensing and apoptotic signaling. Pharmacological inhibition of PERK reduces pathology in neurodegenerative disease models but can cause side effects, indicating the importance of precise regulation. Among ER stress sensors, PERK modulates cell fate decisions, acting as a potential therapeutic target for diseases characterized by ER stress and protein misfolding.

References
https://pubmed.ncbi.nlm.nih.gov/24180212/ https://pubmed.ncbi.nlm.nih.gov/26225772/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%