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Anti-PCBP2 Mouse Antibody [L1D11]

Catalog No.: F4324

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IHC1:120 IF1:40

Application
WB, IHC, IF

Reactivity
Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Observed MW
38.7 Kda
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-PCBP2 Mouse Antibody [L1D11] recognizes endogenous levels of total PCBP2 protein.

Clone
L1D11

Synonym(s)
Poly(rC)-binding protein 2, Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2 (hnRNP E2), PCBP2, HNRPE2, MGC110998.

Background
Poly(C)-binding proteins (Pcbps), also known as hnRNPEs or αCPs, represent a broadly expressed family of RNA-binding proteins (RBPs) with diverse cellular roles. The two principal members are Pcbp1 and Pcbp2. Pcbp1 acts as an intronless paralog of Pcbp2 through retrotransposition of a processed Pcbp2 transcript. While Pcbp1 and Pcbp2 share considerable structural similarity and binding specificity, resulting in overlapping functions, each also carries out distinct isoform-specific roles. Pcbps function at the interface of nuclear and cytoplasmic gene regulation. In the nucleus, they participate in RNA processing events such as splicing and polyadenylation, whereas in the cytoplasm, they modulate mRNA stability and translation. These activities are mediated through recognition of cytosine- and pyrimidine-rich elements in pre-mRNAs and mature transcripts. The regulatory capacity of Pcbps is further shaped by post-translational modifications, subcellular localization, and interactions with metal ions such as iron, zinc, and folate in different biological contexts. Within the immune system, Pcbp2 is broadly expressed in both innate and adaptive compartments. It is indispensable for the proliferation of conventional T (Tconv) cells by regulating co-stimulatory signaling pathways. Pcbp2 activity is therefore required to achieve full activation of Tconv cells in vivo.

Background

Poly(C)-binding proteins (Pcbps), also known as hnRNPEs or αCPs, represent a broadly expressed family of RNA-binding proteins (RBPs) with diverse cellular roles. The two principal members are Pcbp1 and Pcbp2. Pcbp1 acts as an intronless paralog of Pcbp2 through retrotransposition of a processed Pcbp2 transcript. While Pcbp1 and Pcbp2 share considerable structural similarity and binding specificity, resulting in overlapping functions, each also carries out distinct isoform-specific roles. Pcbps function at the interface of nuclear and cytoplasmic gene regulation. In the nucleus, they participate in RNA processing events such as splicing and polyadenylation, whereas in the cytoplasm, they modulate mRNA stability and translation. These activities are mediated through recognition of cytosine- and pyrimidine-rich elements in pre-mRNAs and mature transcripts. The regulatory capacity of Pcbps is further shaped by post-translational modifications, subcellular localization, and interactions with metal ions such as iron, zinc, and folate in different biological contexts. Within the immune system, Pcbp2 is broadly expressed in both innate and adaptive compartments. It is indispensable for the proliferation of conventional T (Tconv) cells by regulating co-stimulatory signaling pathways. Pcbp2 activity is therefore required to achieve full activation of Tconv cells in vivo.

References
https://pubmed.ncbi.nlm.nih.gov/36632062/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%