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Anti-MALT1/MLT Rabbit Antibody [P2K2]

Catalog No.: F2801

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:10000 IP1:50 IF1:250 FCM1:100

Application
WB, IP, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
92 kDa 92 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-MALT1/MLT Rabbit Antibody [P2K2] detects endogenous levels of total MALT1/MLT protein.

Clone
P2K2

Synonym(s)
MLT, MALT1, Mucosa-associated lymphoid tissue lymphoma translocation protein 1, MALT lymphoma-associated translocation, Paracaspase

Background
MALT1/MLT (Mucosa-associated lymphoid tissue lymphoma translocation protein 1), also known as paracaspase 1, is a ubiquitously expressed signaling protein that plays a dual role as a scaffold and arginine-specific protease within immune signaling pathways. Structurally, MALT1 contains an N-terminal death domain (DD), followed by two Ig-like domains, a central paracaspase catalytic domain, and a C-terminal Ig-like domain that stabilizes and regulates protease activity. Unlike classical caspases, MALT1 remains as a single uncleaved polypeptide and is activated by dimerization, with its paracaspase domain structurally resembling caspases but recognizing and cleaving substrates specifically after arginine residues. Functionally, MALT1 is a core component of the CARMA1–BCL10–MALT1 (CBM) signalosome, where it recruits E3 ligases such as TRAF6 and promotes downstream activation of NF-κB and JNK pathways, thereby regulating lymphocyte activation, proliferation, and survival. Through its protease activity, MALT1 cleaves key negative regulators of NF-κB signaling, such as A20 and CYLD, fine-tuning immune responses, while its scaffolding function organizes signaling complexes critical for adaptive immunity and lymphomagenesis.

Background

MALT1/MLT (Mucosa-associated lymphoid tissue lymphoma translocation protein 1), also known as paracaspase 1, is a ubiquitously expressed signaling protein that plays a dual role as a scaffold and arginine-specific protease within immune signaling pathways. Structurally, MALT1 contains an N-terminal death domain (DD), followed by two Ig-like domains, a central paracaspase catalytic domain, and a C-terminal Ig-like domain that stabilizes and regulates protease activity. Unlike classical caspases, MALT1 remains as a single uncleaved polypeptide and is activated by dimerization, with its paracaspase domain structurally resembling caspases but recognizing and cleaving substrates specifically after arginine residues. Functionally, MALT1 is a core component of the CARMA1–BCL10–MALT1 (CBM) signalosome, where it recruits E3 ligases such as TRAF6 and promotes downstream activation of NF-κB and JNK pathways, thereby regulating lymphocyte activation, proliferation, and survival. Through its protease activity, MALT1 cleaves key negative regulators of NF-κB signaling, such as A20 and CYLD, fine-tuning immune responses, while its scaffolding function organizes signaling complexes critical for adaptive immunity and lymphomagenesis.

References
https://pubmed.ncbi.nlm.nih.gov/22309193/ https://pubmed.ncbi.nlm.nih.gov/38863711/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%