Anti-M6PR (cation dependent) Rabbit Antibody [J4M13]

Catalog No.: F2833

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 - 1:10000 IF1:50 - 1:100 FCM1:80

Application
WB, IF, FCM

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
31 kDa

Positive Control	Human uterus; Mouse kidney; Rat kidney; Rat spleen; HAP1 cell; A549 cell; HeLa cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Anti-M6PR (cation dependent) Rabbit Antibody [J4M13] detects endogenous levels of total M6PR protein.

Clone
J4M13

Synonym(s)
MPR46, MPRD, M6PR, Cation-dependent mannose-6-phosphate receptor, CD Man-6-P receptor, CD-MPR, 46 kDa mannose 6-phosphate receptor, MPR 46

Background
M6PR (cation dependent) is also called cation-dependent mannose 6-phosphate receptor (CD-MPR) is a type I transmembrane glycoprotein and a member of the P-type lectin family that plays a central role in lysosomal enzyme trafficking. Structurally, it functions as a homodimer, with each subunit containing a single mannose 6-phosphate (Man-6-P) binding site in its extracytoplasmic domain (residues 1–154), and its binding affinity is modulated by pH and divalent cations. CD-MPR is expressed ubiquitously in mammalian cells, where it cycles between the trans-Golgi network, endosomes, and the plasma membrane. Its primary function is to recognize and bind newly synthesized Man-6-P–tagged acid hydrolases in the mildly acidic environment of the trans-Golgi network (pH ~6.5), transport them to endosomes, and release them under more acidic conditions (

Background

M6PR (cation dependent) is also called cation-dependent mannose 6-phosphate receptor (CD-MPR) is a type I transmembrane glycoprotein and a member of the P-type lectin family that plays a central role in lysosomal enzyme trafficking. Structurally, it functions as a homodimer, with each subunit containing a single mannose 6-phosphate (Man-6-P) binding site in its extracytoplasmic domain (residues 1–154), and its binding affinity is modulated by pH and divalent cations. CD-MPR is expressed ubiquitously in mammalian cells, where it cycles between the trans-Golgi network, endosomes, and the plasma membrane. Its primary function is to recognize and bind newly synthesized Man-6-P–tagged acid hydrolases in the mildly acidic environment of the trans-Golgi network (pH ~6.5), transport them to endosomes, and release them under more acidic conditions (

References
https://pubmed.ncbi.nlm.nih.gov/18272523/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%