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Anti-LAMP1 Rabbit Antibody [L9J5]

Catalog No.: F4075

Application: Reactivity: Human, Monkey

Usage Information

Dilution
WB1:1000 IP1:100 IHC1:100 - 1:400 IF1:100 - 1:400 FCM1:100 - 1:400

Application
WB, IP, IHC, IF, FCM

Reactivity
Human, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
42 kDa(non-glycosylated), 90-120 kDa(glycosylated)

Datasheet & SDS

Biological Description

Specificity
Anti-LAMP1 Rabbit Antibody [L9J5] recognizes endogenous levels of total LAMP1 protein.

Clone
L9J5

Synonym(s)
Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a, LAMP1

Background
Lysosome-associated membrane protein-1 (LAMP1), a member of the LAMP protein family, was originally identified as a marker of mature dendritic cells (CD208, DC-LAMP). Also known as CD107a, LAMP1 plays a critical role in maintaining lysosomal membrane stability, shielding it from intracellular proteolysis, and supporting lysosomal acidification. Its terminal sialyl Lewis X (sLeX) glycan residues mediate interactions with E-selectin-expressing endothelial cells, thereby contributing to cell adhesion, tumor invasion, and metastasis. While LAMP1 is predominantly localized to the endosomal–lysosomal membrane, it can also be detected on the plasma membrane. Notably, high levels of LAMP1 are expressed on the cell surface of metastatic tumor cells, implicating it in cell–cell adhesion and tumor cell migration. Molecular studies further suggest that LAMP1 is actively involved in the development and progression of several cancers, including colorectal cancer, pancreatic carcinoma, ovarian cancer, breast cancer, and esophageal squamous cell carcinoma (ESCC).

Background

Lysosome-associated membrane protein-1 (LAMP1), a member of the LAMP protein family, was originally identified as a marker of mature dendritic cells (CD208, DC-LAMP). Also known as CD107a, LAMP1 plays a critical role in maintaining lysosomal membrane stability, shielding it from intracellular proteolysis, and supporting lysosomal acidification. Its terminal sialyl Lewis X (sLeX) glycan residues mediate interactions with E-selectin-expressing endothelial cells, thereby contributing to cell adhesion, tumor invasion, and metastasis. While LAMP1 is predominantly localized to the endosomal–lysosomal membrane, it can also be detected on the plasma membrane. Notably, high levels of LAMP1 are expressed on the cell surface of metastatic tumor cells, implicating it in cell–cell adhesion and tumor cell migration. Molecular studies further suggest that LAMP1 is actively involved in the development and progression of several cancers, including colorectal cancer, pancreatic carcinoma, ovarian cancer, breast cancer, and esophageal squamous cell carcinoma (ESCC).

References
https://pubmed.ncbi.nlm.nih.gov/35145930/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%