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Anti-LAMC2 Mouse Antibody [J11A23]

Catalog No.: F3711

Application: Reactivity: Human

Usage Information

Dilution
WB1:500 - 1:1000 IHC1:500 - 1:1000 IF1:100 - 1:500

Application
WB, IHC, IF

Reactivity
Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
131 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-LAMC2 Mouse Antibody [J11A23] detetcts endogenous levels of total LAMC2 protein.

Clone
J11A23

Synonym(s)
LAMB2T, LAMNB2, LAMC2, Laminin subunit gamma-2, Cell-scattering factor 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma, CSF 140 kDa subunit

Background
LAMC2 (Laminin subunit γ2) is a heparin binding-associated gene encoding the γ2 chain of laminin-332, a multi-domain extracellular matrix glycoprotein involved in cell–matrix interactions. Unlike other laminin γ chains, LAMC2 lacks domain VI and contains shortened domains V–III, contributing to the unique structure of laminin-332, where the γ2 chain forms part of the long arm and short arms critical for adhesion and signaling. LAMC2 is normally expressed at low levels in epithelial basement membranes but is markedly upregulated in various cancers, including pancreatic, lung, bladder, colorectal, cervical, and cholangiocarcinoma. Functionally, LAMC2 regulates cell adhesion, differentiation, and migration, and plays a key role in epithelial–mesenchymal transition (EMT), invasion, and metastasis. High LAMC2 expression is consistently associated with tumor progression, therapy resistance, and poor prognosis, making it a clinically relevant biomarker and potential therapeutic target.

Background

LAMC2 (Laminin subunit γ2) is a heparin binding-associated gene encoding the γ2 chain of laminin-332, a multi-domain extracellular matrix glycoprotein involved in cell–matrix interactions. Unlike other laminin γ chains, LAMC2 lacks domain VI and contains shortened domains V–III, contributing to the unique structure of laminin-332, where the γ2 chain forms part of the long arm and short arms critical for adhesion and signaling. LAMC2 is normally expressed at low levels in epithelial basement membranes but is markedly upregulated in various cancers, including pancreatic, lung, bladder, colorectal, cervical, and cholangiocarcinoma. Functionally, LAMC2 regulates cell adhesion, differentiation, and migration, and plays a key role in epithelial–mesenchymal transition (EMT), invasion, and metastasis. High LAMC2 expression is consistently associated with tumor progression, therapy resistance, and poor prognosis, making it a clinically relevant biomarker and potential therapeutic target.

References
https://pubmed.ncbi.nlm.nih.gov/38020294/ https://pubmed.ncbi.nlm.nih.gov/8786121/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%