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Anti-Ku70 Rabbit Antibody [A6H2]

Catalog No.: F4068

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:10000 IP1:20 IHC1:100 - 1:250 IF1:100 - 1:250 FCM1:10 - 1:100

Application
WB, IP, IHC, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
70 kDa 70 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human colon carcinoma; Human testis; Human tonsil; HeLa cell; A549 cell; 293T cell; A431 cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Anti-Ku70 Rabbit Antibody [A6H2] recognizes endogenous levels of total Ku70 protein.

Clone
A6H2

Synonym(s)
G22P1, XRCC6, X-ray repair cross-complementing protein 6, 5'-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit

Background
Human Ku70 is a key nuclear protein that plays a central role in the non-homologous end joining (NHEJ) pathway for repairing DNA double-strand breaks. It is encoded by the XRCC6 gene on chromosome 22, while its partner protein Ku80 is encoded by XRCC5 on chromosome 2. Ku70 and Ku80 form a heterodimer, and this interaction is crucial for their stability; loss of one subunit leads to a significant reduction in the intracellular level of the other, indicating that they predominantly exist as a dimeric complex. Together, Ku70/Ku80 (collectively referred to as Ku) and the catalytic subunit DNA-PKcs assemble into the DNA-dependent protein kinase (DNA-PK) complex, which is recruited to double-strand breaks to mediate repair via NHEJ. Although Ku70 is mainly localized in the nucleus, it is first translated in the cytoplasm and subsequently transported into the nucleus, either independently or together with Ku80. Beyond its canonical role in DNA repair, Ku70 also functions as a cytosolic DNA sensor. In this capacity, it activates type III interferon production through the STING–TBK1–IRF3/IRF1/IRF7 signaling axis, thereby linking DNA damage response with innate immune signaling.

Background

Human Ku70 is a key nuclear protein that plays a central role in the non-homologous end joining (NHEJ) pathway for repairing DNA double-strand breaks. It is encoded by the XRCC6 gene on chromosome 22, while its partner protein Ku80 is encoded by XRCC5 on chromosome 2. Ku70 and Ku80 form a heterodimer, and this interaction is crucial for their stability; loss of one subunit leads to a significant reduction in the intracellular level of the other, indicating that they predominantly exist as a dimeric complex. Together, Ku70/Ku80 (collectively referred to as Ku) and the catalytic subunit DNA-PKcs assemble into the DNA-dependent protein kinase (DNA-PK) complex, which is recruited to double-strand breaks to mediate repair via NHEJ. Although Ku70 is mainly localized in the nucleus, it is first translated in the cytoplasm and subsequently transported into the nucleus, either independently or together with Ku80. Beyond its canonical role in DNA repair, Ku70 also functions as a cytosolic DNA sensor. In this capacity, it activates type III interferon production through the STING–TBK1–IRF3/IRF1/IRF7 signaling axis, thereby linking DNA damage response with innate immune signaling.

References
https://pubmed.ncbi.nlm.nih.gov/34746031/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%