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Anti-Integrin α 5 Rabbit Antibody [K23D13]

Catalog No.: F2165

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:10000 IP1:10 - 1:100 IHC1:400 IF1:50 FCM1:20

Application
WB, IP, IHC, IF , FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
115 kDa 19 kDa, 150 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-Integrin α 5 Rabbit Antibody [K23D13] detects endogenous levels of total Integrin α 5 protein.

Clone
K23D13

Synonym(s)
CD49e, FNRA, ITGA5, Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5

Background
Integrin α5 is a type I transmembrane glycoprotein that pairs with β1 to form the α5β1 heterodimeric receptor, primarily recognizing fibronectin, a major extracellular matrix (ECM) component. The α5 subunit contains an N-terminal seven-bladed beta-propeller domain responsible for ligand binding, and a cytoplasmic tail that interacts with intracellular signaling and cytoskeletal proteins, facilitating bidirectional signal transduction. Integrin α5β1 plays a pivotal role in cell adhesion, migration, and ECM organization, thereby influencing processes such as embryonic development, tissue repair, angiogenesis, and tumor progression. Its ligand-binding affinity and activation state are regulated by conformational changes driven by divalent cations (e.g., Mn2+, Mg2+) and interactions with the synergy site on fibronectin. Integrin α5β1 activation promotes focal adhesion assembly, linking the ECM to the actin cytoskeleton and triggering signaling cascades that regulate cell shape, proliferation, and survival via pathways like FAK, Src, and MAPK. Regulation of α5 integrin is finely tuned through inside-out signaling via talin and kindlin binding to its cytoplasmic tail, altering its affinity for fibronectin. Additionally, the interaction with urokinase plasminogen activator receptor (uPAR) modulates its conformation and function, influencing adhesion and migration dynamics. Dysregulation of α5β1 integrin is implicated in pathological conditions including cancer metastasis, fibrosis, and vascular diseases.

Background

Integrin α5 is a type I transmembrane glycoprotein that pairs with β1 to form the α5β1 heterodimeric receptor, primarily recognizing fibronectin, a major extracellular matrix (ECM) component. The α5 subunit contains an N-terminal seven-bladed beta-propeller domain responsible for ligand binding, and a cytoplasmic tail that interacts with intracellular signaling and cytoskeletal proteins, facilitating bidirectional signal transduction. Integrin α5β1 plays a pivotal role in cell adhesion, migration, and ECM organization, thereby influencing processes such as embryonic development, tissue repair, angiogenesis, and tumor progression. Its ligand-binding affinity and activation state are regulated by conformational changes driven by divalent cations (e.g., Mn2+, Mg2+) and interactions with the synergy site on fibronectin. Integrin α5β1 activation promotes focal adhesion assembly, linking the ECM to the actin cytoskeleton and triggering signaling cascades that regulate cell shape, proliferation, and survival via pathways like FAK, Src, and MAPK. Regulation of α5 integrin is finely tuned through inside-out signaling via talin and kindlin binding to its cytoplasmic tail, altering its affinity for fibronectin. Additionally, the interaction with urokinase plasminogen activator receptor (uPAR) modulates its conformation and function, influencing adhesion and migration dynamics. Dysregulation of α5β1 integrin is implicated in pathological conditions including cancer metastasis, fibrosis, and vascular diseases.

References
https://pubmed.ncbi.nlm.nih.gov/15684035/ https://pubmed.ncbi.nlm.nih.gov/33962943/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%