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Anti-Huntingtin Rabbit Antibody [A7N5]

Catalog No.: F4009

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:5000 IHC1:100 IF1:1500

Application
WB, IHC, IF

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
348 kDa 348 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-Huntingtin Rabbit Antibody [A7N5] detects endogenous levels of total Huntingtin protein.

Clone
A7N5

Synonym(s)
HD, IT15, HTT, Huntingtin, Huntington disease protein, HD protein

Background
Huntingtin (HTT) is a large, 348 kDa protein that plays a pivotal role in embryonic development and cellular homeostasis. Its structure is predicted to form an elongated superhelical solenoid, composed of multiple HEAT repeats that mediate protein-protein interactions. HTT consists of N- and C-terminal domains containing these HEAT repeats, linked by a smaller bridge domain, which allows it to function as a dynamic scaffolding protein, influencing various cellular processes. HTT is involved in vesicular transport, endocytosis, autophagy, and transcriptional regulation. It facilitates bidirectional axonal transport of vesicles and organelles through interactions with motor proteins like kinesin and dynein, as well as accessory factors such as Huntingtin-associated protein 1 (HAP1). HTT also regulates synaptic vesicle endocytosis and exocytosis, impacting neurotransmitter release and synaptic function. HTT interacts with transcriptional regulators such as REST/NRSF and CBP, influencing neuronal survival signals and gene expression. HTT’s activity is regulated by phosphorylation at specific residues, like Ser421, which modulates its function in axonal transport. Mutations in HTT, particularly the expansion of the N-terminal polyglutamine tract, lead to a toxic gain-of-function that disrupts HTT’s normal functions, resulting in neurodegeneration and the development of Huntington’s disease.

Background

Huntingtin (HTT) is a large, 348 kDa protein that plays a pivotal role in embryonic development and cellular homeostasis. Its structure is predicted to form an elongated superhelical solenoid, composed of multiple HEAT repeats that mediate protein-protein interactions. HTT consists of N- and C-terminal domains containing these HEAT repeats, linked by a smaller bridge domain, which allows it to function as a dynamic scaffolding protein, influencing various cellular processes. HTT is involved in vesicular transport, endocytosis, autophagy, and transcriptional regulation. It facilitates bidirectional axonal transport of vesicles and organelles through interactions with motor proteins like kinesin and dynein, as well as accessory factors such as Huntingtin-associated protein 1 (HAP1). HTT also regulates synaptic vesicle endocytosis and exocytosis, impacting neurotransmitter release and synaptic function. HTT interacts with transcriptional regulators such as REST/NRSF and CBP, influencing neuronal survival signals and gene expression. HTT’s activity is regulated by phosphorylation at specific residues, like Ser421, which modulates its function in axonal transport. Mutations in HTT, particularly the expansion of the N-terminal polyglutamine tract, lead to a toxic gain-of-function that disrupts HTT’s normal functions, resulting in neurodegeneration and the development of Huntington’s disease.

References
https://pubmed.ncbi.nlm.nih.gov/22180703/ https://pubmed.ncbi.nlm.nih.gov/29466333/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%