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Anti-hnRNP A1 Mouse Antibody [A20P15]

Catalog No.: F4060

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IHC1:2000 FCMUse 1µg for 10⁶ cells

Application
WB, IP, IHC, IF, FCM

Reactivity
Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
38 kDa 37 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-hnRNP A1 Mouse Antibody [A20P15] recognizes endogenous levels of total hnRNP A1 protein.

Clone
A20P15

Synonym(s)
HNRPA1, HNRNPA1, Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1

Background
Eukaryotic cells produce a wide range of RNA-binding proteins (RBPs), each with distinct affinities and specificities for their RNA targets. These proteins are essential regulators of nearly every stage of RNA metabolism, from biogenesis to functional activity. Among them, the heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a large and diverse family of RBPs that carry out multiple roles in the conversion of heterogeneous nuclear RNAs into mature messenger RNAs. HnRNP A1 is one of the most abundant and ubiquitously expressed members of this family. It contributes to gene expression by influencing nearly all major steps of RNA transcript processing, including transcription, splicing, mRNA stability, nuclear export, and translation of both cellular and viral RNAs. Its functions extend beyond mRNA biogenesis to include regulation of microRNA processing, maintenance of telomeres, and modulation of transcription factor activity. Genomic studies have highlighted the broad influence of hnRNP A1 in organismal development and differentiation. Although primarily localized in the nucleus under steady-state conditions, hnRNP A1 is a shuttling protein that can transit between the nucleus and cytoplasm in response to specific signals. Its activity and localization are fine-tuned by post-translational modifications such as methylation, phosphorylation, and SUMOylation. Furthermore, hnRNP A1 directly interacts with transcription factors, regulating their activity through protein–protein interactions.

Background

Eukaryotic cells produce a wide range of RNA-binding proteins (RBPs), each with distinct affinities and specificities for their RNA targets. These proteins are essential regulators of nearly every stage of RNA metabolism, from biogenesis to functional activity. Among them, the heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a large and diverse family of RBPs that carry out multiple roles in the conversion of heterogeneous nuclear RNAs into mature messenger RNAs. HnRNP A1 is one of the most abundant and ubiquitously expressed members of this family. It contributes to gene expression by influencing nearly all major steps of RNA transcript processing, including transcription, splicing, mRNA stability, nuclear export, and translation of both cellular and viral RNAs. Its functions extend beyond mRNA biogenesis to include regulation of microRNA processing, maintenance of telomeres, and modulation of transcription factor activity. Genomic studies have highlighted the broad influence of hnRNP A1 in organismal development and differentiation. Although primarily localized in the nucleus under steady-state conditions, hnRNP A1 is a shuttling protein that can transit between the nucleus and cytoplasm in response to specific signals. Its activity and localization are fine-tuned by post-translational modifications such as methylation, phosphorylation, and SUMOylation. Furthermore, hnRNP A1 directly interacts with transcription factors, regulating their activity through protein–protein interactions.

References
https://pubmed.ncbi.nlm.nih.gov/24065100/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%