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Anti-FOXJ1 Mouse Antibody [P11C9]

Catalog No.: F3932

Application: Reactivity: Human, Mouse

Usage Information

Dilution
WB1:50 - 1:500 IHC1:100 - 1:1000

Application
WB, IHC, IF

Reactivity
Human, Mouse

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
50-kDa

Datasheet & SDS

Biological Description

Specificity
Anti-FOXJ1 Mouse Antibody [P11C9] detects endogenous levels of total FOXJ1 protein.

Clone
P11C9

Synonym(s)
fork head homologue 4; Forkhead box protein J1; forkhead transcription factor HFH-4; forkhead-like 13; Forkhead-related protein FKHL13; Hepatocyte nuclear factor 3 forkhead homolog 4; HFH-4; HNF-3/forkhead homolog 4; MGC35202, FKHL-13; FKHL13; FOXJ1; HFH-4; HFH4

Background
FOXJ1 (Forkhead box protein J1) is a member of the FOX family of transcription factors, characterized by a conserved winged-helix DNA-binding domain that enables regulation of gene expression programs critical for development and disease. It is predominantly expressed in tissues bearing motile cilia, including the respiratory epithelium, ependymal lining of the brain ventricles, and embryonic ventral node, where it acts as a master regulator of ciliogenesis by activating transcription of structural and regulatory ciliary genes. Beyond ciliated tissues, FOXJ1 is also detected in the neural tube floor plate, forebrain, olfactory bulbs, teeth epithelia, and some immune cells, highlighting broader regulatory functions. Functionally, FOXJ1 controls the docking and orientation of basal bodies, axonemal outgrowth, and ciliary motility, and its loss results in defective ciliogenesis, impaired left-right patterning, and developmental abnormalities. In cancer biology, FOXJ1 exhibits tissue-dependent roles: it is frequently downregulated in prostate, breast, and ovarian cancers—suppressing proliferation and invasion via modulation of MMP-2 and MMP-9—while in some contexts like liver cancer it may promote tumor progression. Thus, FOXJ1 serves dual roles as a key transcriptional regulator of motile ciliogenesis and as a context-dependent modulator of tumorigenesis.

Background

FOXJ1 (Forkhead box protein J1) is a member of the FOX family of transcription factors, characterized by a conserved winged-helix DNA-binding domain that enables regulation of gene expression programs critical for development and disease. It is predominantly expressed in tissues bearing motile cilia, including the respiratory epithelium, ependymal lining of the brain ventricles, and embryonic ventral node, where it acts as a master regulator of ciliogenesis by activating transcription of structural and regulatory ciliary genes. Beyond ciliated tissues, FOXJ1 is also detected in the neural tube floor plate, forebrain, olfactory bulbs, teeth epithelia, and some immune cells, highlighting broader regulatory functions. Functionally, FOXJ1 controls the docking and orientation of basal bodies, axonemal outgrowth, and ciliary motility, and its loss results in defective ciliogenesis, impaired left-right patterning, and developmental abnormalities. In cancer biology, FOXJ1 exhibits tissue-dependent roles: it is frequently downregulated in prostate, breast, and ovarian cancers—suppressing proliferation and invasion via modulation of MMP-2 and MMP-9—while in some contexts like liver cancer it may promote tumor progression. Thus, FOXJ1 serves dual roles as a key transcriptional regulator of motile ciliogenesis and as a context-dependent modulator of tumorigenesis.

References
https://pubmed.ncbi.nlm.nih.gov/27914912/ https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=The+expression+and+functional+role+of+FOX+transcription+factor+FOXJ1+in+prostate+cancer&btnG=

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%