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Anti-Endo G Rabbit Antibody [A9E22]

Catalog No.: F3659

Application: Reactivity: Human, Mouse

Usage Information

Dilution
WB1:2000 - 1:5000

Application
WB

Reactivity
Human, Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
36 kDa 35 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Mouse liver; HepG2 cell; HAP1 cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Anti-Endo G Rabbit Antibody [A9E22] detects endogenous levels of total Endonuclease G protein.

Clone
A9E22

Synonym(s)
Endo G, ENDOG

Background
EndoG (Endonuclease G) is an evolutionarily conserved mitochondrial nuclear encoded, sugar-non-specific nuclease that exists predominantly as a homodimer with a ββα-metal finger motif at its active site, enabling cleavage of both DNA and RNA in a sequence-independent manner. Encoded in the nucleus but localized to the mitochondrial intermembrane space, EndoG can be released during apoptosis and translocated into the nucleus, where it contributes to chromosomal DNA fragmentation in a caspase-independent pathway. Beyond apoptosis, EndoG also functions in mitochondrial DNA maintenance, recombination, and the selective degradation of paternal mitochondrial DNA during embryogenesis. Its nuclease activity is tightly regulated by compartmentalization, dimerization, redox state, and specific inhibitors (e.g., EndoGI in Drosophila), ensuring controlled participation in both normal cellular metabolism and programmed cell death.

Background

EndoG (Endonuclease G) is an evolutionarily conserved mitochondrial nuclear encoded, sugar-non-specific nuclease that exists predominantly as a homodimer with a ββα-metal finger motif at its active site, enabling cleavage of both DNA and RNA in a sequence-independent manner. Encoded in the nucleus but localized to the mitochondrial intermembrane space, EndoG can be released during apoptosis and translocated into the nucleus, where it contributes to chromosomal DNA fragmentation in a caspase-independent pathway. Beyond apoptosis, EndoG also functions in mitochondrial DNA maintenance, recombination, and the selective degradation of paternal mitochondrial DNA during embryogenesis. Its nuclease activity is tightly regulated by compartmentalization, dimerization, redox state, and specific inhibitors (e.g., EndoGI in Drosophila), ensuring controlled participation in both normal cellular metabolism and programmed cell death.

References
https://pubmed.ncbi.nlm.nih.gov/19783821/ https://pubmed.ncbi.nlm.nih.gov/27738134/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%