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Anti-DNAJB6 N-terminal Rabbit Antibody [A12P20]

Catalog No.: F3180

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 IP1:150 IHC1:100 IF1:250 FCM1:500

Application
WB, IP, IHC, IF, FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
36 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-DNAJB6 N-terminal Rabbit Antibody [A12P20] detects endogenous levels of total DNAJB6 N-terminal protein.

Clone
A12P20

Synonym(s)
HSJ2, MRJ, MSJ1, DNAJB6, DnaJ homolog subfamily B member 6, HHDJ1, Heat shock protein J2, MSJ-1, HSJ-2

Background
DNAJB6, also referred to as the mammalian relative of DnaJ (MRJ), encodes a highly conserved co-chaperone belonging to the DnaJ/Hsp40 protein family that cooperates with Hsp70 chaperones. It is ubiquitously expressed across tissues, with particularly high levels observed in the brain. DNAJB6 participates in a broad range of cellular processes, including murine placental development, suppression of misfolded protein aggregate formation and toxicity, and regulation of neural stem cell self-renewal. Dysregulation of DNAJB6 has been linked to several diseases, such as Huntington’s disease, Parkinson’s disease, limb-girdle muscular dystrophy, cardiomyocyte hypertrophy, and cancer. Functionally, DNAJB6 suppresses polyglutamine (polyQ)-mediated protein aggregation and mitigates toxicity induced by mutant huntingtin protein, thereby contributing to neuroprotection. DNAJB6 is one of the most abundantly expressed DNAJB family members in skeletal muscle, underscoring its biological significance for muscle function. However, this same importance also makes muscle tissue particularly vulnerable to pathogenic mutations. Mutations associated with DNAJB6-related myopathies act through deleterious gain-of-function mechanisms, driving disease development.

Background

DNAJB6, also referred to as the mammalian relative of DnaJ (MRJ), encodes a highly conserved co-chaperone belonging to the DnaJ/Hsp40 protein family that cooperates with Hsp70 chaperones. It is ubiquitously expressed across tissues, with particularly high levels observed in the brain. DNAJB6 participates in a broad range of cellular processes, including murine placental development, suppression of misfolded protein aggregate formation and toxicity, and regulation of neural stem cell self-renewal. Dysregulation of DNAJB6 has been linked to several diseases, such as Huntington’s disease, Parkinson’s disease, limb-girdle muscular dystrophy, cardiomyocyte hypertrophy, and cancer. Functionally, DNAJB6 suppresses polyglutamine (polyQ)-mediated protein aggregation and mitigates toxicity induced by mutant huntingtin protein, thereby contributing to neuroprotection. DNAJB6 is one of the most abundantly expressed DNAJB family members in skeletal muscle, underscoring its biological significance for muscle function. However, this same importance also makes muscle tissue particularly vulnerable to pathogenic mutations. Mutations associated with DNAJB6-related myopathies act through deleterious gain-of-function mechanisms, driving disease development.

References
https://pubmed.ncbi.nlm.nih.gov/27276715/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%