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Anti-Desmoplakin I+II Mouse Antibody [M24F24]

Catalog No.: F2831

Application: Reactivity: Human, Mouse, Rat, Chicken, Cow

Usage Information

Dilution
WB1:1000

Application
WB, IHC-Fr, IF

Reactivity
Human, Mouse, Rat, Chicken, Cow

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

Biological Description

Specificity
Anti-Desmoplakin I+II Mouse Antibody [M24F24] detects endogenous levels of total Desmoplakin I+II protein.

Clone
M24F24

Synonym(s)
Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen, DSP

Background
Desmoplakin I+II are major high–molecular weight proteins of the desmosomal plaque that function as essential linkers between intermediate filaments (IFs) and cell–cell junctions, thereby providing tissue integrity under mechanical stress. Both isoforms are encoded by the same gene through alternative splicing, with DPI containing a long central α-helical coiled-coil rod domain (~132 nm) and DPII having a shorter rod due to deletion of 599 residues. Their structure is organized into three regions: an N-terminal domain mediating desmosome targeting, a central rod promoting dimerization, and a C-terminal domain with repeated motifs that directly interact with IFs such as keratins, vimentin, and desmin. DPs are highly expressed in desmosome-rich tissues such as epidermis and myocardium, where they stabilize intercellular junctions by anchoring the IF cytoskeleton to the plasma membrane. Functionally, they are crucial for maintaining epithelial and cardiac tissue integrity, and mutations in DP cause skin blistering disorders and cardiomyopathies due to defective IF attachment and desmosomal stability.

Background

Desmoplakin I+II are major high–molecular weight proteins of the desmosomal plaque that function as essential linkers between intermediate filaments (IFs) and cell–cell junctions, thereby providing tissue integrity under mechanical stress. Both isoforms are encoded by the same gene through alternative splicing, with DPI containing a long central α-helical coiled-coil rod domain (~132 nm) and DPII having a shorter rod due to deletion of 599 residues. Their structure is organized into three regions: an N-terminal domain mediating desmosome targeting, a central rod promoting dimerization, and a C-terminal domain with repeated motifs that directly interact with IFs such as keratins, vimentin, and desmin. DPs are highly expressed in desmosome-rich tissues such as epidermis and myocardium, where they stabilize intercellular junctions by anchoring the IF cytoskeleton to the plasma membrane. Functionally, they are crucial for maintaining epithelial and cardiac tissue integrity, and mutations in DP cause skin blistering disorders and cardiomyopathies due to defective IF attachment and desmosomal stability.

References
https://pubmed.ncbi.nlm.nih.gov/1293163/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%