Choose Your Country or Region

Anti-CYP2E1 Mouse Antibody [F19L7]

Catalog No.: F3827

Application: Reactivity: Mouse, Rat, Pig

Usage Information

Dilution
WB1:1000-1:6000 IHC1:500-1:2000 IF1:400-1:1600

Application
WB, IHC, IF, ELISA

Reactivity
Mouse, Rat, Pig

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
57 kDa 50 kDa-57 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-CYP2E1 Mouse Antibody [F19L7] detects endogenous levels of total CYP2E1 protein.

Clone
F19L7

Synonym(s)
CYP2E1, 1F12G7, 4-nitrophenol 2-hydroxylase, CPE1, CYP2E

Background
Cytochrome P450 2E1 (CYP2E1), an ethanol-metabolizing enzyme, is a member of the cytochrome P450 superfamily of heme-containing monooxygenases that catalyze the oxidative metabolism of a wide range of endogenous and exogenous compounds. Structurally, CYP2E1 is an endoplasmic reticulum–anchored enzyme composed of a hydrophobic N-terminal membrane-binding domain and a conserved catalytic heme-binding domain that facilitates electron transfer from NADPH–cytochrome P450 reductase to substrates. It is constitutively expressed at high levels in the liver, but is also found in lung, kidney, brain, nasal mucosa, small intestine, and testes, with expression inducible by ethanol, nicotine, fasting, and various xenobiotics. Functionally, CYP2E1 plays a dual role: it metabolizes physiologic substrates such as fatty acids and ketone bodies, while also bioactivating a variety of drugs (e.g., acetaminophen, chlorzoxazone, halothane) and environmental toxins (e.g., benzene, nitrosamines, 1,3-butadiene) into reactive intermediates. This enzymatic activity contributes to both detoxification and carcinogenesis, as CYP2E1 generates DNA-reactive metabolites and reactive oxygen species (ROS), thereby mediating oxidative stress, lipid peroxidation, and cytotoxicity. Its inducible nature and tissue-specific expression make CYP2E1 a critical determinant of chemical toxicity, cancer risk, and inter-individual variability in drug metabolism.

Background

Cytochrome P450 2E1 (CYP2E1), an ethanol-metabolizing enzyme, is a member of the cytochrome P450 superfamily of heme-containing monooxygenases that catalyze the oxidative metabolism of a wide range of endogenous and exogenous compounds. Structurally, CYP2E1 is an endoplasmic reticulum–anchored enzyme composed of a hydrophobic N-terminal membrane-binding domain and a conserved catalytic heme-binding domain that facilitates electron transfer from NADPH–cytochrome P450 reductase to substrates. It is constitutively expressed at high levels in the liver, but is also found in lung, kidney, brain, nasal mucosa, small intestine, and testes, with expression inducible by ethanol, nicotine, fasting, and various xenobiotics. Functionally, CYP2E1 plays a dual role: it metabolizes physiologic substrates such as fatty acids and ketone bodies, while also bioactivating a variety of drugs (e.g., acetaminophen, chlorzoxazone, halothane) and environmental toxins (e.g., benzene, nitrosamines, 1,3-butadiene) into reactive intermediates. This enzymatic activity contributes to both detoxification and carcinogenesis, as CYP2E1 generates DNA-reactive metabolites and reactive oxygen species (ROS), thereby mediating oxidative stress, lipid peroxidation, and cytotoxicity. Its inducible nature and tissue-specific expression make CYP2E1 a critical determinant of chemical toxicity, cancer risk, and inter-individual variability in drug metabolism.

References
https://pubmed.ncbi.nlm.nih.gov/16368115/ https://pubmed.ncbi.nlm.nih.gov/10418964/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%