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Anti-CPT2 C-terminal Rabbit Antibody [H24H13]

Catalog No.: F2414

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:50 - 1:100 IF1:50 - 1:100

Application
WB, IHC, IF

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
74 kDa 74 kDa, 70 kDa, 67 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human fetal kidney tissue; Mouse heart tissue; Mouse kidney tissue; Rat kidney tissue; Rat liver tissue; Human fetal liver tissue; Human fetal kidney tissue; Human liver tissue; Human skeletal muscle tissue; Human liver carcinoma tissue; Mouse kidney tissue; Rat colon tissue; HeLa cell; HAP1 cell; MCF7 cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Anti-CPT2 C-terminal Rabbit Antibody [H24H13] detects endogenous levels of total C-terminal region of CPT2 protein.

Clone
H24H13

Synonym(s)
CPT1, CPT2, Carnitine palmitoyltransferase II, CPT II

Background
Carnitine palmitoyltransferase 2 (CPT2) is a mitochondrial enzyme located in the inner mitochondrial membrane that plays a crucial role in fatty acid metabolism by converting long-chain acylcarnitines to acyl-CoA, facilitating their entry into the β-oxidation pathway for energy production. CPT2 consists of two major domains: the N-terminal domain and the C-terminal domain, each featuring a six-stranded antiparallel β-sheet flanked by α-helices. The C-terminal domain is vital for substrate specificity, enzyme stability, and membrane association. It forms part of the active site where catalysis occurs and contains hydrophobic patches that mediate its interaction with the mitochondrial membrane and the carnitine-acylcarnitine translocase (CACT), enabling efficient substrate channeling. CPT2 acts as a rate-limiting enzyme in the mitochondrial fatty acid oxidation process, with its activity regulated by substrate availability and membrane interactions. Mutations or dysfunction in CPT2, particularly affecting its C-terminal domain, lead to metabolic disorders like CPT2 deficiency, characterized by impaired fatty acid breakdown, muscle weakness, and metabolic crises.

Background

Carnitine palmitoyltransferase 2 (CPT2) is a mitochondrial enzyme located in the inner mitochondrial membrane that plays a crucial role in fatty acid metabolism by converting long-chain acylcarnitines to acyl-CoA, facilitating their entry into the β-oxidation pathway for energy production. CPT2 consists of two major domains: the N-terminal domain and the C-terminal domain, each featuring a six-stranded antiparallel β-sheet flanked by α-helices. The C-terminal domain is vital for substrate specificity, enzyme stability, and membrane association. It forms part of the active site where catalysis occurs and contains hydrophobic patches that mediate its interaction with the mitochondrial membrane and the carnitine-acylcarnitine translocase (CACT), enabling efficient substrate channeling. CPT2 acts as a rate-limiting enzyme in the mitochondrial fatty acid oxidation process, with its activity regulated by substrate availability and membrane interactions. Mutations or dysfunction in CPT2, particularly affecting its C-terminal domain, lead to metabolic disorders like CPT2 deficiency, characterized by impaired fatty acid breakdown, muscle weakness, and metabolic crises.

References
https://pubmed.ncbi.nlm.nih.gov/16781677/ https://pubmed.ncbi.nlm.nih.gov/19430727/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%