Anti-COX IV Rabbit Antibody [D5A5]

Catalog No.: F0274

Application: Reactivity: Human, Rat, Monkey, Zebrafish, Bovine, Pig

Usage Information

Dilution
WB1:1000 IP1:100 IHC1:500 - 1:2000 IF1:200 - 1:800 FCM1:400 - 1:1600

Application
WB, IP, IHC, IF, FCM

Reactivity
Human, Rat, Monkey, Zebrafish, Bovine, Pig

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
17 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-COX IV Rabbit Antibody [D5A5] detects endogenous levels of total COX IV protein.

Clone
D5A5

Synonym(s)
cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Background
COX IV (Cytochrome c oxidase subunit IV) is a small nuclear-encoded protein that plays a critical regulatory and structural role in mitochondrial complex IV, the terminal enzyme of the electron transport chain. It is synthesized with an N-terminal mitochondrial transit peptide that directs its import into mitochondria, where the mature protein integrates into the cytochrome c oxidase complex near the heme and copper catalytic centers. COX IV exists as two isoforms: COX4I1, the predominant isoform under normoxic conditions, and COX4I2, which is preferentially expressed under hypoxia, allowing adaptive regulation of oxidative phosphorylation in response to oxygen availability. Functionally, COX IV is essential for the proper assembly and allosteric regulation of complex IV, enabling efficient electron transfer from cytochrome c to oxygen and tight control of ATP production. Its expression levels correlate with cellular metabolic activity and energy demands, underscoring its central role in maintaining mitochondrial bioenergetics.

Background

COX IV (Cytochrome c oxidase subunit IV) is a small nuclear-encoded protein that plays a critical regulatory and structural role in mitochondrial complex IV, the terminal enzyme of the electron transport chain. It is synthesized with an N-terminal mitochondrial transit peptide that directs its import into mitochondria, where the mature protein integrates into the cytochrome c oxidase complex near the heme and copper catalytic centers. COX IV exists as two isoforms: COX4I1, the predominant isoform under normoxic conditions, and COX4I2, which is preferentially expressed under hypoxia, allowing adaptive regulation of oxidative phosphorylation in response to oxygen availability. Functionally, COX IV is essential for the proper assembly and allosteric regulation of complex IV, enabling efficient electron transfer from cytochrome c to oxygen and tight control of ATP production. Its expression levels correlate with cellular metabolic activity and energy demands, underscoring its central role in maintaining mitochondrial bioenergetics.

References
https://pubmed.ncbi.nlm.nih.gov/17091399/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%