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Anti-Cdc25C Rabbit Antibody [K18P10]

Catalog No.: F3337

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:5000 IP1:30 - 1:80 IHC1:2500 IF1:250 - 1:500

Application
WB, IP, IHC, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
53 kDa 58 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-Cdc25C Rabbit Antibody [K18P10] detects endogenous levels of total Cdc25C protein.

Clone
K18P10

Synonym(s)
M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C

Background
Cdc25C is a critical dual-specificity phosphatase that regulates cell cycle progression by activating the cyclin B1/CDK1 complex, controlling the G2/M transition and mitotic entry. It is composed of approximately 473 amino acids, organized into an N-terminal regulatory domain and a highly conserved C-terminal catalytic domain containing the HCX5R motif, which is essential for its phosphatase activity. The N-terminal domain contains multiple phosphorylation sites and binding motifs, such as nuclear localization and export sequences, which regulate Cdc25C’s subcellular localization, stability, and activity via interactions with proteins like 14-3-3 and checkpoint kinases. The catalytic domain includes a crucial cysteine residue necessary for substrate dephosphorylation. Cdc25C dephosphorylates Thr14 and Tyr15 on CDK1, promoting the formation of the active cyclin B1/CDK1 complex that is essential for mitotic entry. Its activity is tightly regulated through phosphorylation-dependent mechanisms involving kinases such as CHK1, CHK2, and ATM, especially in response to DNA damage, leading to its inhibition and cell cycle arrest at G2/M to prevent genomic instability. Cdc25C is also regulated by interactions with 14-3-3 proteins and can be inactivated by kinases like Wee1 and Myt1. Cdc25C modulates apoptosis pathways by negatively regulating the ASK1 kinase during interphase, thus preventing premature apoptosis. Dysregulation or overexpression of Cdc25C has been implicated in cancer, where it contributes to uncontrolled cell proliferation and evasion of cell cycle checkpoints.

Background

Cdc25C is a critical dual-specificity phosphatase that regulates cell cycle progression by activating the cyclin B1/CDK1 complex, controlling the G2/M transition and mitotic entry. It is composed of approximately 473 amino acids, organized into an N-terminal regulatory domain and a highly conserved C-terminal catalytic domain containing the HCX5R motif, which is essential for its phosphatase activity. The N-terminal domain contains multiple phosphorylation sites and binding motifs, such as nuclear localization and export sequences, which regulate Cdc25C’s subcellular localization, stability, and activity via interactions with proteins like 14-3-3 and checkpoint kinases. The catalytic domain includes a crucial cysteine residue necessary for substrate dephosphorylation. Cdc25C dephosphorylates Thr14 and Tyr15 on CDK1, promoting the formation of the active cyclin B1/CDK1 complex that is essential for mitotic entry. Its activity is tightly regulated through phosphorylation-dependent mechanisms involving kinases such as CHK1, CHK2, and ATM, especially in response to DNA damage, leading to its inhibition and cell cycle arrest at G2/M to prevent genomic instability. Cdc25C is also regulated by interactions with 14-3-3 proteins and can be inactivated by kinases like Wee1 and Myt1. Cdc25C modulates apoptosis pathways by negatively regulating the ASK1 kinase during interphase, thus preventing premature apoptosis. Dysregulation or overexpression of Cdc25C has been implicated in cancer, where it contributes to uncontrolled cell proliferation and evasion of cell cycle checkpoints.

References
https://pubmed.ncbi.nlm.nih.gov/10864927/ https://pubmed.ncbi.nlm.nih.gov/32518522/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%