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Anti-CD74 Rabbit Antibody [K17P4]

Catalog No.: F4094

Application: Reactivity: Human, Monkey

Usage Information

Dilution
WB1:1000 IHC1:250 - 1:1000 IF1:200 FCM1:400 - 1:1600

Application
WB, IHC, IF, FCM

Reactivity
Human, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
20-25 kDa, 30-40 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-CD74 Rabbit Antibody [K17P4] recognizes endogenous levels of total CD74 protein.

Clone
K17P4

Synonym(s)
HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain (Ii), CD74, DHLAG

Background
CD74, also known as the invariant chain (Ii) of the major histocompatibility complex class II (MHC II), is a type II transmembrane glycoprotein that plays a central role in antigen presentation by serving as a chaperone for MHC II molecules. Its expression is primarily found in antigen-presenting cells such as B cells, monocytes/macrophages, and dendritic cells, though it is also present in T cells. Traditionally, CD74 is recognized for its function in facilitating the assembly and intracellular trafficking of MHC II complexes. Beyond its canonical role in antigen presentation, CD74 also participates in a range of MHC II–independent processes. It regulates endosomal trafficking and cell migration, and at the cell surface, it functions as a high-affinity receptor for macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine that acts as an upstream regulator in cardiovascular, infectious, autoimmune, and malignant diseases. CD74’s interaction with MIF initiates signaling cascades that modulate inflammation and cellular responses. In cancer biology, CD74 is frequently upregulated and has been linked to increased tumor proliferation and metastatic behavior. It is also expressed in several hematological malignancies, including multiple myeloma. Structurally, CD74 is a single-pass transmembrane protein with type II topology, placing its N-terminus in the cytoplasm. Through its diverse roles, CD74 influences signal transduction, membrane trafficking, and migration in antigen-presenting cells, while also contributing to disease pathogenesis and tumor progression.

Background

CD74, also known as the invariant chain (Ii) of the major histocompatibility complex class II (MHC II), is a type II transmembrane glycoprotein that plays a central role in antigen presentation by serving as a chaperone for MHC II molecules. Its expression is primarily found in antigen-presenting cells such as B cells, monocytes/macrophages, and dendritic cells, though it is also present in T cells. Traditionally, CD74 is recognized for its function in facilitating the assembly and intracellular trafficking of MHC II complexes. Beyond its canonical role in antigen presentation, CD74 also participates in a range of MHC II–independent processes. It regulates endosomal trafficking and cell migration, and at the cell surface, it functions as a high-affinity receptor for macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine that acts as an upstream regulator in cardiovascular, infectious, autoimmune, and malignant diseases. CD74’s interaction with MIF initiates signaling cascades that modulate inflammation and cellular responses. In cancer biology, CD74 is frequently upregulated and has been linked to increased tumor proliferation and metastatic behavior. It is also expressed in several hematological malignancies, including multiple myeloma. Structurally, CD74 is a single-pass transmembrane protein with type II topology, placing its N-terminus in the cytoplasm. Through its diverse roles, CD74 influences signal transduction, membrane trafficking, and migration in antigen-presenting cells, while also contributing to disease pathogenesis and tumor progression.

References
https://pubmed.ncbi.nlm.nih.gov/27033518/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%