Anti-Blimp-1/PRDI-BF1 Rabbit Antibody [K23C1]

Catalog No.: F0425

Application: Reactivity: Human, Mouse

Usage Information

Dilution
WB1:1000 IP1:100 IF1:100 FCM1:50

Application
WB, IP, IF, FCM

Reactivity
Human, Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
95 kDa-100 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-Blimp-1/PRDI-BF1 Rabbit Antibody [K23C1] detects endogenous levels of total Blimp-1/PRDI-BF1 protein.

Clone
K23C1

Synonym(s)
PRDM1

Background
Blimp-1/PRDI-BF1, is a 789-amino-acid transcriptional repressor that contains five carboxy-terminal zinc finger motifs mediating sequence-specific DNA binding. It is expressed in activated B cells and sustained in plasma cells, where it functions as a master regulator of terminal B-cell differentiation by repressing genes such as c-myc to halt proliferation and promote plasma cell identity. Beyond B cells, PRDI-BF1 is inducible by viral infection and represses the interferon-β (IFN-β) promoter, ensuring transient cytokine expression. Mechanistically, its amino-terminal repression domain actively silences transcription by recruiting Groucho/TLE family corepressors, enabling long-range repression. Thus, PRDI-BF1/Blimp-1 integrates developmental and antiviral programs by enforcing terminal differentiation, regulating apoptosis, and modulating immune gene expression.

Background

Blimp-1/PRDI-BF1, is a 789-amino-acid transcriptional repressor that contains five carboxy-terminal zinc finger motifs mediating sequence-specific DNA binding. It is expressed in activated B cells and sustained in plasma cells, where it functions as a master regulator of terminal B-cell differentiation by repressing genes such as c-myc to halt proliferation and promote plasma cell identity. Beyond B cells, PRDI-BF1 is inducible by viral infection and represses the interferon-β (IFN-β) promoter, ensuring transient cytokine expression. Mechanistically, its amino-terminal repression domain actively silences transcription by recruiting Groucho/TLE family corepressors, enabling long-range repression. Thus, PRDI-BF1/Blimp-1 integrates developmental and antiviral programs by enforcing terminal differentiation, regulating apoptosis, and modulating immune gene expression.

References
https://pubmed.ncbi.nlm.nih.gov/9887105/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%