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Anti-Ataxin 3 Rabbit Antibody [K3D4]

Catalog No.: F4066

Application: Reactivity: Mouse, Human, Rat

Usage Information

Dilution
WB1:1000 IHC1:100 IF1:500

Application
WB, IHC, IF

Reactivity
Mouse, Human, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
42 kDa

Positive Control	Mouse stomach; Human lung; Mouse liver; Rat liver; MCF-7 cell; HEK-293T cell
Negative Control	Rat brain

Datasheet & SDS

Biological Description

Specificity
Anti-Ataxin 3 Rabbit Antibody [K3D4] recognizes endogenous levels of total Ataxin 3 protein.

Clone
K3D4

Synonym(s)
ATX3, MJD, MJD1, SCA3, ATXN3, Ataxin-3, Machado-Joseph disease protein 1, Spinocerebellar ataxia type 3 protein

Background
Ataxin-3 is a widely expressed deubiquitinating enzyme that plays key roles in the ubiquitin–proteasome degradation pathway and in the regulation of transcription. The protein has a molecular weight of about 42 kDa and is most abundantly expressed in the brain, although it is present in many tissues. Its C-terminal region contains a polyglutamine (PolyQ) tract, and expansion of this tract beyond 52 glutamines results in the neurodegenerative disorder spinocerebellar ataxia type 3 (SCA3). Ataxin-3 is distributed throughout the cell and is capable of shuttling between the cytoplasm and nucleus. Its subcellular localization is determined by different protein domains and can be dynamically regulated. Functionally active ataxin-3 tends to accumulate in the nucleus more frequently than inactive forms. Nuclear import is further modulated by phosphorylation events: the protein contains six potential CK2 phosphorylation sites and one site targeted by GSK3β. Phosphorylation of serines 236, 340, and 352 by CK2 specifically promotes nuclear localization. Beyond localization control, ataxin-3 also regulates its own ubiquitination and modulates the stability and function of E3 ubiquitin ligases such as CHIP (C-terminus of Hsp70-interacting protein) and Parkin through its deubiquitinase activity. Thus, ataxin-3 is a well-characterized regulator of the ubiquitin–proteasome system, with additional roles in the degradation of misfolded endoplasmic reticulum (ER) proteins.

Background

Ataxin-3 is a widely expressed deubiquitinating enzyme that plays key roles in the ubiquitin–proteasome degradation pathway and in the regulation of transcription. The protein has a molecular weight of about 42 kDa and is most abundantly expressed in the brain, although it is present in many tissues. Its C-terminal region contains a polyglutamine (PolyQ) tract, and expansion of this tract beyond 52 glutamines results in the neurodegenerative disorder spinocerebellar ataxia type 3 (SCA3). Ataxin-3 is distributed throughout the cell and is capable of shuttling between the cytoplasm and nucleus. Its subcellular localization is determined by different protein domains and can be dynamically regulated. Functionally active ataxin-3 tends to accumulate in the nucleus more frequently than inactive forms. Nuclear import is further modulated by phosphorylation events: the protein contains six potential CK2 phosphorylation sites and one site targeted by GSK3β. Phosphorylation of serines 236, 340, and 352 by CK2 specifically promotes nuclear localization. Beyond localization control, ataxin-3 also regulates its own ubiquitination and modulates the stability and function of E3 ubiquitin ligases such as CHIP (C-terminus of Hsp70-interacting protein) and Parkin through its deubiquitinase activity. Thus, ataxin-3 is a well-characterized regulator of the ubiquitin–proteasome system, with additional roles in the degradation of misfolded endoplasmic reticulum (ER) proteins.

References
https://pubmed.ncbi.nlm.nih.gov/24293103/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%