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Anti-ACO2 Rabbit Antibody [L24N14]

Catalog No.: F4090

Application: Reactivity: Human, Mouse, Rat, Hamster, Monkey

Usage Information

Dilution
WB1:1000 IP1:50 IF1:200

Application
WB, IP, IF

Reactivity
Human, Mouse, Rat, Hamster, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
85 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-ACO2 Rabbit Antibody [L24N14] recognizes endogenous levels of total ACO2 protein.

Clone
L24N14

Synonym(s)
Aconitate hydratase, mitochondrial, Aconitase, Citrate hydro-lyase, ACO2

Background
Aconitase 2 (ACO2) is an iron–sulfur [4Fe-4S] cluster enzyme belonging to the hydrase family, where the [4Fe-4S] cofactor, together with structural elements of the protein, catalyzes the reversible isomerization of citrate to isocitrate in the tricarboxylic acid (TCA) cycle through sequential dehydration and rehydration steps. ACO2 is essential for cellular energy production, iron homeostasis, oxidative stress defense, and the preservation of mitochondrial DNA (mtDNA) integrity. Aconitase 2 (ACO2) is an iron–sulfur [4Fe-4S] cluster enzyme belonging to the hydrase family, where the [4Fe-4S] cofactor, together with structural elements of the protein, catalyzes the reversible isomerization of citrate to isocitrate in the tricarboxylic acid (TCA) cycle through sequential dehydration and rehydration steps. ACO2 is essential for cellular energy production, iron homeostasis, oxidative stress defense, and the preservation of mitochondrial DNA (mtDNA) integrity. The enzyme’s activity is tightly regulated by multiple mechanisms, most notably the oxidative damage of its [4Fe-4S] cluster by reactive oxygen species (ROS), post-translational modifications, and genetic variations that alter its function.

Background

Aconitase 2 (ACO2) is an iron–sulfur [4Fe-4S] cluster enzyme belonging to the hydrase family, where the [4Fe-4S] cofactor, together with structural elements of the protein, catalyzes the reversible isomerization of citrate to isocitrate in the tricarboxylic acid (TCA) cycle through sequential dehydration and rehydration steps. ACO2 is essential for cellular energy production, iron homeostasis, oxidative stress defense, and the preservation of mitochondrial DNA (mtDNA) integrity. Aconitase 2 (ACO2) is an iron–sulfur [4Fe-4S] cluster enzyme belonging to the hydrase family, where the [4Fe-4S] cofactor, together with structural elements of the protein, catalyzes the reversible isomerization of citrate to isocitrate in the tricarboxylic acid (TCA) cycle through sequential dehydration and rehydration steps. ACO2 is essential for cellular energy production, iron homeostasis, oxidative stress defense, and the preservation of mitochondrial DNA (mtDNA) integrity. The enzyme’s activity is tightly regulated by multiple mechanisms, most notably the oxidative damage of its [4Fe-4S] cluster by reactive oxygen species (ROS), post-translational modifications, and genetic variations that alter its function.

References
https://pubmed.ncbi.nlm.nih.gov/38007539/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%