PMA (Phorbol 12-myristate 13-acetate)

Catalog No.S7791 Batch:S779103

Technical Data

Formula

C₃₆H₅₆O₈

Molecular Weight

616.83

CAS No.

16561-29-8

Solubility (25°C)*

In vitro

DMSO

100 mg/mL (162.11 mM)

Ethanol

100 mg/mL (162.11 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution

5%DMSO 1 Corn oil

5.0mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	PMA (Phorbol 12-myristate 13-acetate), a potent activator of PKC, is active at nanomolar concentrations. Phorbol 12-myristate 13-acetate (PMA) induces sphingosine-1-phosphate (S1P).PMA has strong excitability on the skin and mucous membrane. Special protection is required when using PMA. Wear gloves and mask to avoid direct contact in any way.
In vitro	In endothelial cells, PMA increases levels of Thy-1 mRNA and protein, induces Thy-1 up-regulation and inhibits capillary-like tube formation and endothelial cell migration.^[1]
In vivo	In the zebrafish model, PMA initially enhances endothelial cell migration, subsequently activates the PKC-δ/Syk/NF-κB-mediated pathway to up-regulate Thy-1, which in turn inhibits endothelial cell migration.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Human umbilical vein endothelial cells (HUVECs), human dermal microvascular endothelial cells (HDMECs), human pulmonary microvascular endothelial cell line (HPMECs) Concentrations 20 ng/mL Incubation Time 14, 16 and 18 h for levels of Ty-1 mRNA, 20, 24 and 28 h for levels of Ty-1 protein Method After transfection with Ty-1 promoter plasmid (0.5 μg) and Renilla plasmid (50ng), the cells are treated with PMA (20ng/mL), and washed with ice-cold phosphate-buffered saline (PBS), and then lysed in passive lysis buffer. Luciferase activity is measured using the Dual-Luciferase Reporter Assay kit.
Animal Study:^{^[1]}	Animal Models Transgenic Tg(fi1:EGFP)y1 embryos of zebrafsh (Danio rerio) Dosages 20 ng/mL Administration --

Cell Assay:^{^[1]}

Cell lines
Human umbilical vein endothelial cells (HUVECs), human dermal microvascular endothelial cells (HDMECs), human pulmonary microvascular endothelial cell line (HPMECs)
Concentrations
20 ng/mL
Incubation Time
14, 16 and 18 h for levels of Ty-1 mRNA, 20, 24 and 28 h for levels of Ty-1 protein
Method
After transfection with Ty-1 promoter plasmid (0.5 μg) and Renilla plasmid (50ng), the cells are treated with PMA (20ng/mL), and washed with ice-cold phosphate-buffered saline (PBS), and then lysed in passive lysis buffer. Luciferase activity is measured using the Dual-Luciferase Reporter Assay kit.

Animal Study:^{^[1]}

Animal Models
Transgenic Tg(fi1:EGFP)y1 embryos of zebrafsh (Danio rerio)
Dosages
20 ng/mL
Administration
--

References

[1] Wen HC, et al. Sci Rep. 2018 Nov 2;8(1):16247.

Selleck's PMA (Phorbol 12-myristate 13-acetate) has been cited by 54 publications

GPR65 sensing tumor-derived lactate induces HMGB1 release from TAM via the cAMP/PKA/CREB pathway to promote glioma progression [ J Exp Clin Cancer Res, 2024, 43(1):105]	PubMed: 38576043
CircMLH3 induces mononuclear macrophage pyroptosis in sepsis by sponging miR-590-3p to regulate TAK1 expression [ Int J Biol Macromol, 2024, 263(Pt 1):130179]	PubMed: 38378118
ALIX and TSG101 are essential for cellular entry and replication of two porcine alphacoronaviruses [ PLoS Pathog, 2024, 20(3):e1012103]	PubMed: 38489378
Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer [ Cancer Commun (Lond), 2023, 43(8):909-937]	PubMed: 37434399
Targeting TCF19 sensitizes MSI endometrial cancer to anti-PD-1 therapy by alleviating CD8+ T cell exhaustion via TRIM14-IFN-β axis [ Cell Rep, 2023, 42(8):112944]	PubMed: 37566545
Transferred mitochondria accumulate reactive oxygen species, promoting proliferation [ Elife, 2023, 12e85494]	PubMed: 36876914
Transferred mitochondria accumulate reactive oxygen species, promoting proliferation [ Elife, 2023, 10.7554/eLife.85494]	PubMed: 36876914
Regulation of PD-L1 expression in non-small cell lung cancer by interleukin-1β [ Front Immunol, 2023, 14:1192861]	PubMed: 37441079
Stimulation of antitumor immunity by FoxP3-targeting PROTAC [ Biomed Pharmacother, 2023, 163:114871]	PubMed: 37182514
Tumor-derived interleukin-1 receptor antagonist exhibits immunosuppressive functions and promotes pancreatic cancer [ Cell Biosci, 2023, 13(1):147]	PubMed: 37563620

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.