MCC950

Catalog No.S8930 Batch:S893003

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Technical Data

Formula

C20H24N2O5S
Molecular Weight 404.48 CAS No. 210826-40-7
Solubility (25°C)* In vitro DMSO 80 mg/mL (197.78 mM)
Ethanol 5 mg/mL (12.36 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.05mg/ml Taking the 1 mL working solution as an example, add 50 μL of 81 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
0.35mg/ml Taking the 1 mL working solution as an example, add 50 μL of 7 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description MCC950 (CP-456773, CRID3) is a potent and selective inhibitor of NLRP3 (NOD-like receptor (NLR) family, pyrin domain-containing protein 3) with IC50 of 7.5 nM and 8.1 nM in BMDMs and HMDMs, respectively.
Targets
NLRP3 [1]
(BMDM-based assay)		 NLRP3 [1]
(HMDM-based assay)
7.5 nM 8.1 nM

Biological Activity

Description MCC950 (CP-456773, CRID3) is a potent and selective inhibitor of NLRP3 (NOD-like receptor (NLR) family, pyrin domain-containing protein 3) with IC50 of 7.5 nM and 8.1 nM in BMDMs and HMDMs, respectively.
Targets
NLRP3 [1]
(BMDM-based assay)		 NLRP3 [1]
(HMDM-based assay)
7.5 nM 8.1 nM
In vitro

MCC950 specifically inhibits NLPR3 inflammasome activation.[1]
In vivo

Inhibition of NLRP3 with MCC950 ameliorates proteinuria, renal histological lesions and podocyte foot process effacement in lupus-prone mice.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    NZM2328 podocyte cell line

  • Concentrations

    1 µM

  • Incubation Time

    24 h

  • Method

    The NZM2328 podocyte cell line was stimulated with a control serum or a serum from diseased NZM2328 mice or serum from diseased NZM2328 mice depleted of IgG, with or without MCC950 or Mito TEMPO for 24 hours. After this incubation, cells were subjected to flow cytometry and Western blot analyses for NLRP3 inflammasome activation, mitochondrial membrane potential and production of reactive oxygen species (ROS) analysis.

    (Data sourced from selleck products)
Animal Study:

[1]
  • Animal Models

    female NZM2328, a new NZM strain produced by extended backcrosses of NZB/WF1 to NZW mice

  • Dosages

    10mg/kg

  • Administration

    i.p.

    (Data sourced from selleck products)

Selleck's MCC950 has been cited by 100 publications

METTL14 contributes to acute lung injury by stabilizing NLRP3 expression in an IGF2BP2-dependent manner [ Cell Death Dis, 2024, 15(1):43] PubMed: 38218935
ChemR23 signaling ameliorates brain injury via inhibiting NLRP3 inflammasome-mediated neuronal pyroptosis in ischemic stroke [ J Transl Med, 2024, 22(1):23] PubMed: 38178174
Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner [ Cell Death Discov, 2024, 10(1):122] PubMed: 38458999
Melatonin alleviates chronic stress-induced hippocampal microglia pyroptosis and subsequent depression-like behaviors by inhibiting Cathepsin B/NLRP3 signaling pathway in rats [ Transl Psychiatry, 2024, 14(1):166] PubMed: 38538614
From inflammation to pyroptosis: Understanding the consequences of cadmium exposure in chicken liver cells [ Ecotoxicol Environ Saf, 2024, 272:116004] PubMed: 38290315
GLUT1 promotes NLRP3 inflammasome activation of airway epithelium in lipopolysaccharide-induced acute lung injury [ Am J Pathol, 2024, S0002-9440(24)00116-0] PubMed: 38548270
NLRP3 inflammasome mediates abnormal epithelial regeneration and distal lung remodeling in silica‑induced lung fibrosis [ Int J Mol Med, 2024, 53(3)25] PubMed: 38240085
Poly-(ADP-ribose) polymerases inhibition by olaparib attenuates activities of the NLRP3 inflammasome and of NF-κB in THP-1 monocytes [ PLoS One, 2024, 19(2):e0295837] PubMed: 38335214
CB2R activation ameliorates late adolescent chronic alcohol exposure-induced anxiety-like behaviors during withdrawal by preventing morphological changes and suppressing NLRP3 inflammasome activation in prefrontal cortex microglia in mice [ Brain Behav Immun, 2023, 110:60-79] PubMed: 36754245
Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing [ Cell Rep Med, 2023, 4(8):101129] PubMed: 37480849

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.