Puromycin 2HCl

Catalog No.S7417 Batch:S7417

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Technical Data

Formula

C22H29N7O5.2HCl
Molecular Weight 544.43 CAS No. 58-58-2
Solubility (25°C)* In vitro DMSO 100 mg/mL warmed with 50ºC water bath (183.67 mM)
Water 100 mg/mL warmed with 50ºC water bath (183.67 mM)
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation.
In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1]

Puromycin can be used for the selection of recombinant cells from noncultured cells. [2]
In vivo

Puromycin (aminonucleoside) inhibits protein synthesis leading to proteinuria & glomerulosclerosis/nephrosis.[4]

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    HepG2 cells 

  • Concentrations

    1.6 μM

  • Incubation Time

    72 h

  • Method

    Cells were treated with different concentrations of drug for 72 h.
Animal Study:

[4]
  • Animal Models

    Male Sprague-Dawley rats

  • Dosages

    5 mg/100 g

  • Administration

    i.v.

References

  • https://pubmed.ncbi.nlm.nih.gov/14211122/
  • https://pubmed.ncbi.nlm.nih.gov/15385422/
  • https://pubmed.ncbi.nlm.nih.gov/28971619/
  • https://pubmed.ncbi.nlm.nih.gov/3953770/
  • https://pubmed.ncbi.nlm.nih.gov/32435426/
  • https://pubmed.ncbi.nlm.nih.gov/11397778/

Selleck's Puromycin 2HCl has been cited by 224 publications

Single-cell transcriptional dissection illuminates an evolution of immunosuppressive microenvironment during pancreatic ductal adenocarcinoma metastasis [ Signal Transduct Target Ther, 2025, 10(1):182] PubMed: 40484878
IGF2BP1 restricts the induction of human primordial germ cell fate in an m6A-dependent manner [ Cell Stem Cell, 2025, S1934-5909(25)00181-X] PubMed: 40436019
RNA-binding proteins DND1 and NANOS3 cooperatively suppress the entry of germ cell lineage [ Nat Commun, 2025, 16(1):4792] PubMed: 40410171
SAMD9 senses cytosolic double-stranded nucleic acids in epithelial and mesenchymal cells to induce antiviral immunity [ Nat Commun, 2025, 16(1):3756] PubMed: 40263291
Homeodomain protein PRRX1 anchors the Ku heterodimers at DNA double-strand breaks to promote nonhomologous end-joining [ Nucleic Acids Res, 2025, 53(6)gkaf200] PubMed: 40114375
The deubiquitinase USP24 suppresses ferroptosis in triple-negative breast cancer by stabilizing DHODH protein [ Cell Death Dis, 2025, 16(1):564] PubMed: 40715045
Chidamide and cytarabine synergistically treat acute myeloid leukemia: inhibiting ribosome biogenesis via the MYC-RRP9 pathway [ Cell Death Dis, 2025, 16(1):601] PubMed: 40781078
USP9X integrates TGF-β and hypoxia signalings to promote ovarian cancer chemoresistance via HIF-2α-maintained stemness [ Cell Death Dis, 2025, 16(1):312] PubMed: 40246814
NF-κB-mediated EAAT3 upregulation in antioxidant defense and ferroptosis sensitivity in lung cancer [ Cell Death Dis, 2025, 16(1):124] PubMed: 39987248
The m5C methyltransferase NSUN2 promotes progression of acute myeloid leukemia by regulating serine metabolism [ Cell Rep, 2025, 44(5):115661] PubMed: 40343793

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.