S100 Antibody [H19D4]

Catalog No.: F2209

For research use only.

    Home Primary Antibodies S100 Antibody [H19D4]

    Application: Reactivity:

    Usage Information

    Dilution
    1:10 - 1:50
    Application
    IHC
    Reactivity
    Human
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    11 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    S100 Antibody [H19D4] detects endogenous levels of total S100 protein.
    Clone
    H19D4
    Synonym(s)
    S100A; S100A1; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1
    Background
    The S100 protein family is a large group of low-molecular-weight calcium-binding proteins (9–14 kDa) unique to vertebrates, first named for their solubility in 100% ammonium sulfate at neutral pH. They are defined by their conserved structure containing two helix–loop–helix motifs, known as EF-hands, which mediate Ca²⁺ and transition metal (Zn²⁺, Cu²⁺, Mn²⁺) binding. These motifs are linked by a flexible hinge region, while sequence variability in the C-terminal extension and hinge contributes to their diverse functions. S100 proteins typically form homo- or heterodimers, a requirement for their full activation. In humans, at least 21–30 isoforms exist, with most clustered on chromosome 1q21 (epidermal differentiation complex) and others dispersed across chromosomes 4, 5, 21, and X. Their expression is cell- and tissue-specific, reflecting highly specialized and often non-redundant functions. Functionally, S100 proteins act both intracellularly and extracellularly: within cells they regulate transcription, cytoskeleton dynamics, calcium homeostasis, energy metabolism, and apoptosis, while secreted S100 proteins bind to receptors such as RAGE and TLR4, triggering NF-κB, JAK-STAT, and MAPK signaling to drive inflammation, immune activation, and tumor progression. Collectively, S100 proteins are multifunctional regulators of cell growth, differentiation, motility, immune response, and stress adaptation, and their dysregulated expression is strongly associated with cancer, inflammatory disorders, and drug resistance.
    References
    • https://pubmed.ncbi.nlm.nih.gov/35892571/
    • https://pubmed.ncbi.nlm.nih.gov/34485305/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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