Myelin Oligodendrocyte Glycoprotein Antibody [J5E10]

Catalog No.: F2680

For research use only.

    Home Primary Antibodies Myelin Oligodendrocyte Glycoprotein Antibody [J5E10]

    Application: Reactivity:

    Experiment Essentials

    WB
    Recommended wet transfer conditions: 200 mA, 60 min.

    Usage Information

    Dilution
    1:2000
    1:50
    1:650
    Application
    WB, IHC, IF, FCM
    Reactivity
    Mouse, Rat
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    28 kDa
    Positive Control Mouse brain; Rat brain; LN18 MOG Cell
    Negative Control

    Datasheet & SDS

    Biological Description

    Specificity
    Myelin Oligodendrocyte Glycoprotein Antibody [J5E10] detects endogenous levels of total Myelin Oligodendrocyte Glycoprotein protein.
    Subcellular Location
    Cell membrane, Membrane
    Uniprot ID
    Q16653
    Clone
    J5E10
    Synonym(s)
    MOG
    Background
    Myelin oligodendrocyte glycoprotein (MOG) is a minor but highly specific myelin protein of the immunoglobulin (Ig) superfamily, exclusively expressed in the central nervous system (CNS) on the outermost surface of myelin sheaths and oligodendrocyte processes, making it readily accessible to immune surveillance. Structurally, MOG is a glycoprotein composed of a single extracellular Ig variable (IgV) domain, a transmembrane region, a cytoplasmic loop, a membrane-associated segment, and a cytoplasmic tail that regulates intracellular localization and signaling. The human MOG gene is located on chromosome 6 within the HLA locus, and its alternatively spliced isoforms are expressed in a developmentally regulated manner: full-length variants appear during fetal stages, while other isoforms are expressed postnatally, localizing either to the cell surface, endoplasmic reticulum, endocytic compartments, or even in secreted form. Functionally, MOG is thought to act as a cell adhesion molecule, a regulator of oligodendrocyte microtubule stability, and a mediator of myelin–immune system interactions, particularly complement activation. Its extracellular topology makes it a key autoantigen in inflammatory demyelinating disorders, where antibodies against MOG are implicated in multiple sclerosis-like pathology, acute disseminated encephalomyelitis (ADEM), neuromyelitis optica spectrum disorders (NMOSD), optic neuritis, and myelitis. Thus, MOG serves as both a marker of oligodendrocyte maturation and a central molecule at the interface of myelin biology and autoimmunity.
    References
    • https://pubmed.ncbi.nlm.nih.gov/9886048/
    • https://pubmed.ncbi.nlm.nih.gov/28533781/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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