Home Primary Antibodies cGAS Antibody [L20A17]

research use only

cGAS Antibody [L20A17]

Catalog No.: F4205

    Application: Reactivity:
    • F4205-wb
      Lane 1: 3T3, Lane 2: Neuro-2a, Lane 3: C2C12

    Usage Information

    Dilution
    1:1000
    1:200
    Application
    WB, IP
    Reactivity
    Mouse
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    62 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    cGAS Antibody [L20A17] detects endogenous levels of total cGAS protein.
    Clone
    L20A17
    Synonym(s)
    Cyclic GMP-AMP synthase; cGAMP synthase; cGAS; m-cGAS; 2'3'-cGAMP synthase; Mab-21 domain-containing protein 1; Cgas; Mb21d1
    Background
    Cyclic GMP-AMP synthase (cGAS) is a central cytosolic DNA sensor of the CD-NTase superfamily, essential for innate immune defense. Human cGAS consists of a positively charged N-terminal domain that mediates DNA binding and promotes liquid-liquid phase separation with DNA, thereby protecting DNA from degradation and enhancing cGAS enzymatic activity. The C-terminal catalytic domain contains the NTase core and the conserved Mab21 domain, featuring motifs such as the α-region, KRKR-loop, and KKH-loop, which are critical for DNA recognition and activation. Upon DNA binding, cGAS undergoes dimerization and conformational changes that enable synthesis of the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP. cGAMP subsequently binds to the ER adaptor protein STING, triggering its dimerization, structural rearrangement, and translocation from the ER to the Golgi via the COPII complex. At the Golgi, STING is phosphorylated by TBK1, creating a signaling platform that recruits and activates IRF3 and NF-κB, leading to the induction of type I interferons and inflammatory cytokines crucial for antiviral immunity. The ability of cGAS to phase-separate with DNA amplifies signaling and prevents premature DNA degradation. Nuclear cGAS activity is tightly controlled by nucleosome binding, which inhibits activation and prevents autoimmunity. Dysregulation of the cGAS-STING pathway is implicated in autoimmune diseases such as lupus, and the pathway plays context-dependent, dual roles in tumorigenesis.
    References
    • https://pubmed.ncbi.nlm.nih.gov/35536585/
    • https://pubmed.ncbi.nlm.nih.gov/35485309/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

    * Indicates a Required Field

    Please enter your name.
    Please enter your email. Please enter a valid email address.
    Please write something to us.