Anti-StAR Rabbit Antibody [D13A17]

Catalog No.: F0712

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IP1:50 IF1:100 - 1:200

Application
WB, IP, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
28 kDa

Positive Control	Human adrenal; Mouse adrenal; Rat adrenal; MLTC-1 cell; C2C12 cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Anti-StAR Rabbit Antibody [D13A17] detects endogenous levels of total StAR protein.

Clone
D13A17

Synonym(s)
Steroidogenic acute regulatory protein, mitochondrial, START domain-containing protein 1 (StARD1), STARD1

Background
StAR (Steroidogenic acute regulatory protein) is a preprotein synthesized in the cytoplasm and processed into a mature form after cleavage of its N-terminal mitochondrial import sequence. Encoded by the STAR gene, it is highly expressed in steroidogenic tissues such as the adrenal cortex, gonads, and also in the brain, where it may regulate neurosteroid production. Structurally, StAR consists of an N-terminal domain required for mitochondrial targeting and a C-terminal domain critical for its biological activity. Functionally, StAR plays an essential role in steroid hormone biosynthesis by facilitating the transfer of cholesterol from the outer to the inner mitochondrial membrane, where the cholesterol side-chain cleavage enzyme P450scc catalyzes the first, rate-limiting step of steroidogenesis. Mutations in the STAR gene cause congenital lipoid adrenal hyperplasia, underscoring its indispensable role in adrenal and gonadal steroid production.

Background

StAR (Steroidogenic acute regulatory protein) is a preprotein synthesized in the cytoplasm and processed into a mature form after cleavage of its N-terminal mitochondrial import sequence. Encoded by the STAR gene, it is highly expressed in steroidogenic tissues such as the adrenal cortex, gonads, and also in the brain, where it may regulate neurosteroid production. Structurally, StAR consists of an N-terminal domain required for mitochondrial targeting and a C-terminal domain critical for its biological activity. Functionally, StAR plays an essential role in steroid hormone biosynthesis by facilitating the transfer of cholesterol from the outer to the inner mitochondrial membrane, where the cholesterol side-chain cleavage enzyme P450scc catalyzes the first, rate-limiting step of steroidogenesis. Mutations in the STAR gene cause congenital lipoid adrenal hyperplasia, underscoring its indispensable role in adrenal and gonadal steroid production.

References
https://pubmed.ncbi.nlm.nih.gov/8943003/ https://pubmed.ncbi.nlm.nih.gov/19321517/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%