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Anti-RIP3 Rabbit Antibody [H22B6]

Catalog No.: F4226

Application: Reactivity: Mouse

Usage Information

Dilution
WB1:1000 IP1:100 IF1:400 - 1:1600 FCM1:800 - 1:1600

Application
WB, IP, IF, FCM

Reactivity
Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
46-62 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-RIP3 Rabbit Antibody [H22B6] detects endogenous levels of total RIP3 protein.

Clone
H22B6

Synonym(s)
Receptor-interacting serine/threonine-protein kinase 3, RIP-like protein kinase 3, Receptor-interacting protein 3, Ripk3, Rip3

Background
RIP3 (Receptor-interacting protein kinase 3) is a serine/threonine kinase that plays a central role in necroptosis, a regulated form of cell death activated in response to death receptor signaling, particularly by tumor necrosis factor (TNF). RIP3 contains an N-terminal kinase domain similar to RIP1 and a unique C-terminal RIP homotypic interaction motif (RHIM), which is critical for interacting with RIP1 to form the necrosome complex. Upon activation, RIP3 binds to RIP1 via its RHIM domains, leading to autophosphorylation and the subsequent recruitment and phosphorylation of MLKL (mixed lineage kinase domain-like pseudokinase). MLKL then induces necroptotic cell death by causing plasma membrane permeabilization. RIP3 is also involved in regulating processes like inflammasome activation, cytokine production, ROS generation, autophagy, and cell proliferation. RIP3's function is tightly controlled by mechanisms such as phosphorylation and ubiquitination, which modulate its kinase activity and protein stability. Interaction with RIP1 through RHIM motifs is essential for forming the necrosome; mutations in these motifs disrupt complex formation, leading to impaired necroptosis. Dysregulation of RIP3 activity has been implicated in various pathological conditions, including inflammatory diseases, ischemia-reperfusion injury, and neurodegeneration.

Background

RIP3 (Receptor-interacting protein kinase 3) is a serine/threonine kinase that plays a central role in necroptosis, a regulated form of cell death activated in response to death receptor signaling, particularly by tumor necrosis factor (TNF). RIP3 contains an N-terminal kinase domain similar to RIP1 and a unique C-terminal RIP homotypic interaction motif (RHIM), which is critical for interacting with RIP1 to form the necrosome complex. Upon activation, RIP3 binds to RIP1 via its RHIM domains, leading to autophosphorylation and the subsequent recruitment and phosphorylation of MLKL (mixed lineage kinase domain-like pseudokinase). MLKL then induces necroptotic cell death by causing plasma membrane permeabilization. RIP3 is also involved in regulating processes like inflammasome activation, cytokine production, ROS generation, autophagy, and cell proliferation. RIP3's function is tightly controlled by mechanisms such as phosphorylation and ubiquitination, which modulate its kinase activity and protein stability. Interaction with RIP1 through RHIM motifs is essential for forming the necrosome; mutations in these motifs disrupt complex formation, leading to impaired necroptosis. Dysregulation of RIP3 activity has been implicated in various pathological conditions, including inflammatory diseases, ischemia-reperfusion injury, and neurodegeneration.

References
https://pubmed.ncbi.nlm.nih.gov/36224345/ https://pubmed.ncbi.nlm.nih.gov/31198981/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%