Anti-Jagged2 Rabbit Antibody [D13N13]

Catalog No.: F2257

Application: Reactivity: Human, Rat

Usage Information

Dilution
WB1:1000 IP1:50

Application
WB, IP

Reactivity
Human, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
150 kDa

Positive Control	HeLa cell; SK-OV-3 cell; SR cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Anti-Jagged2 Rabbit Antibody [D13N13] detects endogenous levels of total Jagged2 protein. It does not cross-react with Jagged1.

Clone
D13N13

Synonym(s)
HJ2; Jag2; Jagged-2; Notch

Background
Jagged2 is a canonical mammalian Notch ligand belonging to the Serrate/Jagged family, characterized by an N-terminal C2 lipid-binding domain, a receptor-binding DSL domain, and multiple EGF repeats that mediate interactions with Notch receptors. Structurally, Jagged2 shares homology with Jagged1 but differs in its signaling strength and sensitivity to glycosylation modifications such as Fringe-mediated GlcNAc addition. It is expressed in various embryonic and adult tissues, particularly at sites of epithelial–mesenchymal interactions and within the hematopoietic niche. Functionally, Jagged2 activates Notch1 and Notch2, promoting T-cell lineage differentiation while inhibiting B-cell and myeloid development, thereby contributing to developmental processes and fine-tuning hematopoietic lineage outcomes through ligand-specific Notch signaling strength and receptor selectivity.

Background

Jagged2 is a canonical mammalian Notch ligand belonging to the Serrate/Jagged family, characterized by an N-terminal C2 lipid-binding domain, a receptor-binding DSL domain, and multiple EGF repeats that mediate interactions with Notch receptors. Structurally, Jagged2 shares homology with Jagged1 but differs in its signaling strength and sensitivity to glycosylation modifications such as Fringe-mediated GlcNAc addition. It is expressed in various embryonic and adult tissues, particularly at sites of epithelial–mesenchymal interactions and within the hematopoietic niche. Functionally, Jagged2 activates Notch1 and Notch2, promoting T-cell lineage differentiation while inhibiting B-cell and myeloid development, thereby contributing to developmental processes and fine-tuning hematopoietic lineage outcomes through ligand-specific Notch signaling strength and receptor selectivity.

References
https://pubmed.ncbi.nlm.nih.gov/21372153/ https://pubmed.ncbi.nlm.nih.gov/28572448/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%