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Anti-Histone H3 (mono methyl Lys79) Rabbit Antibody [P1J18]

Catalog No.: F4035

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:20000 IHC1:5000 - 1:10000 IF1:2000 - 1:5000

Application
WB, IHC, IF, ChIP

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
15 kDa 15 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human skin; Mouse cerebral cortex; Rat colon cortex; Hela cell; NIH/3T3 cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Anti-Histone H3 (mono methyl Lys79) Rabbit Antibody [P1J18] recognizes endogenous levels of total Histone H3 protein, only upon mono-methylation at Lys79.

Clone
P1J18

Synonym(s)
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J

Background
The nucleosome, consisting of four core histone proteins (H2A, H2B, H3, and H4), serves as the fundamental structural unit of chromatin. Once regarded merely as a static scaffold for DNA compaction, histones are now recognized as dynamic regulators, undergoing diverse post-translational modifications such as acetylation, phosphorylation, methylation, and ubiquitination. Among these, histone methylation is particularly critical, as it governs the establishment of active and repressive chromatin states and plays an essential role in genome programming during development. Arginine methylation on histones H3 (Arg2, Arg17, Arg26) and H4 (Arg3) promotes transcriptional activation, catalyzed by protein arginine methyltransferases (PRMTs), including the transcriptional co-activators PRMT1 and CARM1 (PRMT4). In contrast, lysine methylation occurs mainly at residues H3 (Lys4, Lys9, Lys27, Lys36, Lys79) and H4 (Lys20), where it can mediate both transcriptional activation and repression. These methylation marks act as docking sites for chromatin-modifying complexes, recruiting proteins with methyl-lysine recognition modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD40 domains (WDR5).

Background

The nucleosome, consisting of four core histone proteins (H2A, H2B, H3, and H4), serves as the fundamental structural unit of chromatin. Once regarded merely as a static scaffold for DNA compaction, histones are now recognized as dynamic regulators, undergoing diverse post-translational modifications such as acetylation, phosphorylation, methylation, and ubiquitination. Among these, histone methylation is particularly critical, as it governs the establishment of active and repressive chromatin states and plays an essential role in genome programming during development. Arginine methylation on histones H3 (Arg2, Arg17, Arg26) and H4 (Arg3) promotes transcriptional activation, catalyzed by protein arginine methyltransferases (PRMTs), including the transcriptional co-activators PRMT1 and CARM1 (PRMT4). In contrast, lysine methylation occurs mainly at residues H3 (Lys4, Lys9, Lys27, Lys36, Lys79) and H4 (Lys20), where it can mediate both transcriptional activation and repression. These methylation marks act as docking sites for chromatin-modifying complexes, recruiting proteins with methyl-lysine recognition modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD40 domains (WDR5).

References
https://pubmed.ncbi.nlm.nih.gov/15268870/ https://pubmed.ncbi.nlm.nih.gov/15479858/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%