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Anti-Histone H3 (di methyl Lys36) Rabbit Antibody [H14D5]

Catalog No.: F0429

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IHC1:50 IF1:800 - 1:1600 FCM1:50

Application
WB, IHC, IF, FCM

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
17 kDa

Positive Control	Human gastric carcinoma; HeLa cell; NIH/3T3 cell; C6 cell; COS cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Anti-Histone H3 (di methyl Lys36) Rabbit Antibody [H14D5] detects endogenous levels of histone H3.1, histone H3.2, and histone H3.3, only when di-methylated on Lys36.

Clone
H14D5

Synonym(s)
DNA methylation; H3.1; H3.2; H3K36; H3K36me2; Histone; histone modification; methyl histone

Background
Histone H3 (di methyl Lys36) (H3K36me2) is a post-translational modification located within the globular core domain of histone H3, near the start of an α-helix, and is catalyzed primarily by SET domain–containing methyltransferases such as NSD1, NSD2, NSD3, and ASH1L. Structurally, this mark resides within nucleosomal DNA where access is regulated by chromatin conformation. H3K36me2 is broadly distributed across chromatin in diverse cell types, enriched in gene bodies and enhancer regions, where it plays a critical role in maintaining active transcription. Functionally, it prevents the spread of repressive H3K27me3 deposited by polycomb complexes, recruits DNA methyltransferases such as DNMT3A to shape DNA methylation patterns, and helps direct histone deacetylase complexes like Rpd3S to suppress cryptic transcription initiation. Dysregulation of H3K36me2, through altered expression or mutation of its methyltransferases, is strongly linked to developmental syndromes and multiple cancers due to widespread reprogramming of gene expression.

Background

Histone H3 (di methyl Lys36) (H3K36me2) is a post-translational modification located within the globular core domain of histone H3, near the start of an α-helix, and is catalyzed primarily by SET domain–containing methyltransferases such as NSD1, NSD2, NSD3, and ASH1L. Structurally, this mark resides within nucleosomal DNA where access is regulated by chromatin conformation. H3K36me2 is broadly distributed across chromatin in diverse cell types, enriched in gene bodies and enhancer regions, where it plays a critical role in maintaining active transcription. Functionally, it prevents the spread of repressive H3K27me3 deposited by polycomb complexes, recruits DNA methyltransferases such as DNMT3A to shape DNA methylation patterns, and helps direct histone deacetylase complexes like Rpd3S to suppress cryptic transcription initiation. Dysregulation of H3K36me2, through altered expression or mutation of its methyltransferases, is strongly linked to developmental syndromes and multiple cancers due to widespread reprogramming of gene expression.

References
https://pubmed.ncbi.nlm.nih.gov/19155214/ https://pubmed.ncbi.nlm.nih.gov/34782608/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%