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Anti-CD206/MRC1 Rabbit Antibody [K13P18]

Catalog No.: F4083

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IP1:200 IHC1:200 - 1:800 IF1:400 - 1:1600

Application
WB, IP, IHC, IF

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
190-250 kDa

Positive Control	Mouse liver; Mouse spleen; Mouse lung; Mouse bone marrow; Mouse testis; Mouse heart; Normal rhesus monkey spleen; Rat liver; Rat spleen; Mouse colon; Mouse pancreas; 4T1 syngeneic mammary tumor; CT26.WT syngeneic tumor; MUTZ-3 cell
Negative Control	Mouse brain; Rat brain; Rajl cell; Jurkat cell

Datasheet & SDS

Biological Description

Specificity
Anti-CD206/MRC1 Rabbit Antibody [K13P18] detects endogenous levels of total CD206/MRC1 protein.

Clone
K13P18

Synonym(s)
Macrophage mannose receptor 1, MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor (hMR), Macrophage mannose receptor 1-like protein 1, CD206, MRC1, CLEC13D, CLEC13DL, MRC1L1

Background
CD206, also known as the mannose receptor or MRC1, is a type I transmembrane C-type lectin receptor predominantly expressed on macrophages, immature dendritic cells, and certain endothelial cells, playing a crucial role in immune surveillance and homeostasis. CD206 comprises an N-terminal cysteine-rich domain, a fibronectin type II domain, and eight C-type lectin carbohydrate recognition domains (CRDs) that mediate high-affinity binding to glycans containing terminal mannose, fucose, and N-acetylglucosamine residues. CD206 recognizes and internalizes a wide range of endogenous glycoproteins and pathogenic microorganisms through clathrin-mediated endocytosis, facilitating antigen uptake, clearance of glycoproteins, and modulation of immune responses, including the promotion of anti-inflammatory signaling and tissue repair. Regulation of CD206 expression and activity is tightly linked to macrophage polarization, being upregulated in alternatively activated (M2) macrophages by cytokines such as IL-4 and IL-13, and downregulated under pro-inflammatory conditions. Additionally, post-translational modifications like glycosylation influence receptor stability and trafficking, while pH-dependent conformational changes in endosomal compartments facilitate ligand release and receptor recycling. CD206 is dynamically regulated and acts as a vital mediator of immune homeostasis, pathogen recognition, and inflammation resolution.

Background

CD206, also known as the mannose receptor or MRC1, is a type I transmembrane C-type lectin receptor predominantly expressed on macrophages, immature dendritic cells, and certain endothelial cells, playing a crucial role in immune surveillance and homeostasis. CD206 comprises an N-terminal cysteine-rich domain, a fibronectin type II domain, and eight C-type lectin carbohydrate recognition domains (CRDs) that mediate high-affinity binding to glycans containing terminal mannose, fucose, and N-acetylglucosamine residues. CD206 recognizes and internalizes a wide range of endogenous glycoproteins and pathogenic microorganisms through clathrin-mediated endocytosis, facilitating antigen uptake, clearance of glycoproteins, and modulation of immune responses, including the promotion of anti-inflammatory signaling and tissue repair. Regulation of CD206 expression and activity is tightly linked to macrophage polarization, being upregulated in alternatively activated (M2) macrophages by cytokines such as IL-4 and IL-13, and downregulated under pro-inflammatory conditions. Additionally, post-translational modifications like glycosylation influence receptor stability and trafficking, while pH-dependent conformational changes in endosomal compartments facilitate ligand release and receptor recycling. CD206 is dynamically regulated and acts as a vital mediator of immune homeostasis, pathogen recognition, and inflammation resolution.

References
https://www.molbiolcell.org/doi/full/10.1091/mbc.E24-08-0355 https://pubmed.ncbi.nlm.nih.gov/34721436/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%