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Anti-ARNT/HIF-1 β Mouse Antibody [G7C12]

Catalog No.: F3809

Application: Reactivity: Human, Mouse, Rat, Bovine, Ferret, Primate, Sheep

Usage Information

Dilution
WB1:500 IHC1:100 CHIP1:10-1:500

Application
WB, IP, IHC, IF, ChIP

Reactivity
Human, Mouse, Rat, Bovine, Ferret, Primate, Sheep

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
86.6 kDa 92 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human glioblastoma; MDA-MB-231 cell; HeLa cell; HEK293 cell; HASMC cell; HUVEC cell; 786-VHL cell; 786 mock cell; SH-SY5Y cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Anti-ARNT/HIF-1 β Mouse Antibody [G7C12] detects endogenous levels of total ARNT/HIF-1 β protein.

Clone
G7C12

Synonym(s)
ARNT protein, ARNT, aryl hydrocarbon receptor nuclear translocator, BHLHE2, Class E Basic Helix-Loop-Helix Protein 2, Dioxin Receptor, Nuclear Translocator, HIF1 beta, HIF-1 beta, HIF1B, HIF1BETA, HIF-1beta, HIF-1-beta, HIF1-beta, hypoxia-inducible factor 1, beta subunit, Hypoxia-inducible factor 1-beta, TANGO

Background
ARNT/HIF-1 β, is a ubiquitously expressed transcription factor belonging to the basic helix–loop–helix Per-ARNT-Sim (bHLH-PAS) family. Structurally, ARNT contains a bHLH domain for DNA binding, two PAS domains (PAS-A and PAS-B) that mediate dimerization, a nuclear localization signal (NLS) for nuclear import, and a C-terminal transactivation domain. Unlike the oxygen-regulated HIF-α subunits, ARNT is generally considered constitutively expressed, though some tumor cells can upregulate it under hypoxia. Functionally, ARNT serves as an essential dimerization partner for multiple signal-regulated bHLH-PAS proteins, most notably the HIF-α subunits (HIF-1α, HIF-2α, HIF-3α) to form the HIF-1 complex, which drives transcription of hypoxia-responsive genes, and the aryl hydrocarbon receptor (AhR), which mediates xenobiotic responses. Through these partnerships, ARNT plays a pivotal role in both hypoxia adaptation and environmental stress signaling, making it a key integrator of cellular responses to oxygen tension and toxic exposures.

Background

ARNT/HIF-1 β, is a ubiquitously expressed transcription factor belonging to the basic helix–loop–helix Per-ARNT-Sim (bHLH-PAS) family. Structurally, ARNT contains a bHLH domain for DNA binding, two PAS domains (PAS-A and PAS-B) that mediate dimerization, a nuclear localization signal (NLS) for nuclear import, and a C-terminal transactivation domain. Unlike the oxygen-regulated HIF-α subunits, ARNT is generally considered constitutively expressed, though some tumor cells can upregulate it under hypoxia. Functionally, ARNT serves as an essential dimerization partner for multiple signal-regulated bHLH-PAS proteins, most notably the HIF-α subunits (HIF-1α, HIF-2α, HIF-3α) to form the HIF-1 complex, which drives transcription of hypoxia-responsive genes, and the aryl hydrocarbon receptor (AhR), which mediates xenobiotic responses. Through these partnerships, ARNT plays a pivotal role in both hypoxia adaptation and environmental stress signaling, making it a key integrator of cellular responses to oxygen tension and toxic exposures.

References
https://pubmed.ncbi.nlm.nih.gov/24849811/ https://pubmed.ncbi.nlm.nih.gov/9027737/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%