Trabectedin

Synonyms: Ecteinascidin 743, ET-743

Trabectedin (Ecteinascidin 743, ET-743) is a marine alkaloid isolated from the Caribbean tunicate Ecteinascidia turbinata with antitumour activity. Trabectedin binds to the N2 of guanine in the minor groove, causing DNA damage and affecting transcription regulation in a promoter- and gene-specific manner.

Trabectedin Chemical Structure

Trabectedin Chemical Structure

CAS No. 114899-77-3

Purity & Quality Control

Batch: S775801 DMSO]100 mg/mL]false]]]false]]]false Purity: 99.92%
99.92

Trabectedin Related Products

Biological Activity

Description Trabectedin (Ecteinascidin 743, ET-743) is a marine alkaloid isolated from the Caribbean tunicate Ecteinascidia turbinata with antitumour activity. Trabectedin binds to the N2 of guanine in the minor groove, causing DNA damage and affecting transcription regulation in a promoter- and gene-specific manner.
In vitro
In vitro

Trabectedin can block EWS-FLI1 transcription factor activity, suppress Werner syndrome protein (WRN) expression and selectively sensitize Ewing sarcoma cells to the DNA-damaging effects of SN38. <sup><a class="sref" href="#s_ref">[1]</a></sup><sup><a class="sref" href="#s_ref">[3]</a></sup>

Cell Research Cell lines Ewing sarcoma cells
Concentrations 10 nM
Incubation Time 18 h
Method

RNAiMax Lipofectamine (4.5 mL per well in a 6-well dish) was added to siRNA targeting the EWS-FLI1 breakpoint site II, complexed, combined with cells, and incubated for 36 to 48 hours before collection for Western blot analysis.

In Vivo
In vivo

In Tumor-Bearing Mice, trabectedin does not have an impact on the functional activity of MDSC but does significantly reduce the number of Ly6C<sup>high</sup> cells, the most suppressive subset. <sup><a class="sref" href="#s_ref">[4]</a></sup>

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06050434 Recruiting
Soft Tissue Sarcoma
Institut für Klinische Krebsforschung IKF GmbH at Krankenhaus Nordwest|PharmaMar
October 31 2023 --
NCT05868889 Completed
Ovarian Cancer
Grupo Español de Investigación en Cáncer de Ovario
October 21 2021 --
NCT05597917 Recruiting
Soft Tissue Sarcoma
Universität Münster|Anturec Pharmaceuticals GmbH
October 26 2021 Phase 3
NCT04979442 Terminated
Dedifferentiated Liposarcoma
Rain Oncology Inc
July 14 2021 Phase 3

Chemical Information & Solubility

Molecular Weight 761.84 Formula

C39H43N3O11S

CAS No. 114899-77-3 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (131.26 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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