Home Transmembrane Transporters Others Pitstop 2 For research use only.

Pitstop 2

Pitstop 2 is an inhibitor of the interaction of amphiphysin with the amino terminal domain of clathrin that inhibits both clathrin-dependent endocytosis (CDE) and clathrin independent endocytosis (CIE).

Pitstop 2 Chemical Structure

Pitstop 2 Chemical Structure

CAS: 1332879-52-3

Selleck's Pitstop 2 has been cited by 6 publications

Purity & Quality Control

Batch: S967001 DMSO] 95 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 98.92%
98.92

Pitstop 2 Related Products

Biological Activity

Description Pitstop 2 is an inhibitor of the interaction of amphiphysin with the amino terminal domain of clathrin that inhibits both clathrin-dependent endocytosis (CDE) and clathrin independent endocytosis (CIE).
Targets
CIE [1]
In vitro
In vitro

Pitstop 2 is an inhibitor of the interaction of amphiphysin with the amino terminal domain of clathrin and inhibits clathrin-dependent endocytosis (CDE) and clathrin independent endocytosis (CIE) in cells. The effects of pitstop 2 are not restricted to inhibition of clathrin since knockdown of clathrin fails to rescue the inhibition of endocytosis of CIE proteins by the drug. Thus pitstop 2 has additional cellular targets besides the amino terminal domain of clathrin and thus cannot be used to distinguish CIE from CDE.[1]
Cell Research Cell lines Hela cells
Concentrations 20 μM
Incubation Time 15 min
Method

Hela cells are preincubated with DMSO (control) or 20 μM pitstop 2 or pitstop 2-negative control for 15 min. Cells are allowed to internalize Alexa594-Transferrin and antibodies to MHCI for 30 min at 37℃ in the presence of DMSO or drugs. Surface bound antibodies are removed by low pH wash to visualize the internalized proteins (Internal) prior to fixation or cells are fixed immediately after the internalization to label for the internal and surface pools (Total) of the protein. Cells are then labeled with secondary antibodies to detect MHCI.

Chemical Information & Solubility

Molecular Weight 473.36 Formula

C20H13BrN2O3S2
CAS No. 1332879-52-3 SDF --
Smiles BrC1=CC=C(C=C1)\C=C2/SC(=NC2=O)N[S](=O)(=O)C3=CC=CC4=C3C=CC=C4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 95 mg/mL ( (200.69 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Pitstop 2 | Pitstop 2 supplier | purchase Pitstop 2 | Pitstop 2 cost | Pitstop 2 manufacturer | order Pitstop 2 | Pitstop 2 distributor