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Guanidine HCl

Name: Guanidine HCl
Brand: Selleck Chemicals
SKU: S4070
Rating: 5 (1 reviews)

Synonyms: Aminoformamidine HCl

Catalog No.S4070 Purity: 100.00%

Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.

Guanidine HCl Chemical Structure

CAS: 50-01-1

Selleck's Guanidine HCl has been cited by 1 publication

Purity & Quality Control

Batch: S407001 DMSO] 19 mg/mL] false] Water] 19 mg/mL] false] Ethanol] 6 mg/mL] false Purity: 100.00%

100.00

Guanidine HCl Related Products

Biological Activity

Description	Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.

In vitro
In vitro	Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by Guanidine HCl provides several important parameters regarding the mechanism of conformational stability of proteins. ^[1] Guanidine HCl at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. Guanidine HCl (> 1 M) causes co-operative unfolding of the molten globule state. ^[2] Guanidine HCl is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △G^H₂O_app. values of Guanidine HCl are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. ^[3] Guanidine HCl at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI⁺] element from yeast cells. 5 mM Guanidine HCl in growth media cures [PSI⁺] and other prions of yeast. ^[4] 5 mM Guanidine HCl significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine HCl also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase. ^[5]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT03658902	Completed	Botulism; Poisoning	Centre Hospitalier le Mans	January 1 2000	--

References

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Chemical Information & Solubility

Molecular Weight	95.53	Formula	CH₅N₃.HCl
CAS No.	50-01-1	SDF	Download Guanidine HCl SDF
Smiles	C(=N)(N)N.Cl
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 19 mg/mL ( (198.89 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 19 mg/mL

Ethanol : 6 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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