Name: 5-hydroxytryptophan (5-HTP)
Brand: Selleck Chemicals
SKU: S2374
Availability: InStock
Rating: 5 (4 reviews)

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Quality Control

Batch: Purity: 99.73%

99.73

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Solubility

In vitro
Batch:

DMSO : 44 mg/mL (199.8 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 7 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.2mg/ml (9.99mM)	Taking the 1 mL working solution as an example, add 50 μL of 44 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.31mg/ml (1.41mM)	Taking the 1 mL working solution as an example, add 50 μL of 6.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	220.22	Formula	C₁₁H₁₂N₂O₃	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	56-69-9	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC-92523			SMILES	C1=CC2=C(C=C1O)C(=CN2)CC(C(=O)O)N

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro	5-Hydroxytryptophan (5-HTP) is the intermediate metabolite of the essential amino acid L-tryptophan (LT) in the biosynthesis of serotonin. It easily crosses the blood-brain barrier and effectively increases central nervous system (CNS) synthesis of serotonin. Therapeutic administration of this compound has been shown to be effective in treating a wide variety of conditions, including depression, fibromyalgia, binge eating associated with obesity, chronic headaches, and insomnia. Clinical available evidence suggests it is better than placebo at alleviating depression.
In vivo	LD50 of 5-hydroxytryptophan (5-HTP): Mice 1800mg/kg (i.g.); Rats 300mg/kg (i.g.)
References	[1] https://pubmed.ncbi.nlm.nih.gov/9727088/ [2] https://pubmed.ncbi.nlm.nih.gov/11869656/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-08-27)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05895747	RECRUITING	Major Depressive Disorder	University of Utah	2023-09-28	PHASE2
NCT07777328	RECRUITING	Attention Deficit and Hyperactivity Disorder (ADHD); Attention Deficit Hyperactivity Disorder in Adults; Attention Deficits	University of Sheffield	2025-11-25
NCT06718452	NOT_YET_RECRUITING	Tinnitus; Neuroinflammation	University of Campania Luigi Vanvitelli	2026-04-20
NCT04520178	RECRUITING	Spinal Cord Injuries	University of Alberta	2020-07-01	PHASE2; PHASE3
NCT04160910	RECRUITING	Mild or Moderate Asthma With Allergic Sensitization	Indiana University	2021-02-11	PHASE2
NCT04395183	COMPLETED	Major Depressive Disorder	University of Utah	2021-03-01	PHASE2

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5-hydroxytryptophan (5-HTP)

Chemical Structure

Molecular Weight: 220.22