Blebbistatin

Blebbistatin is a selective inhibitor of non-muscle myosin II (NMII). It also potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. It promotes directional migration of corneal endothelial cells (CECs) and accelerates wound healing, and better preserves cell junctional integrity and barrier function.

Blebbistatin Chemical Structure

Blebbistatin Chemical Structure

CAS No. 674289-55-5

Purity & Quality Control

Batch: E124901 DMSO]58 mg/mL]false]Ethanol]5 mg/mL]false]Water]Insoluble]false Purity: 99.39%
99.39

Blebbistatin Related Products

Biological Activity

Description Blebbistatin is a selective inhibitor of non-muscle myosin II (NMII). It also potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. It promotes directional migration of corneal endothelial cells (CECs) and accelerates wound healing, and better preserves cell junctional integrity and barrier function.

Chemical Information & Solubility

Molecular Weight 292.33 Formula

C18H16N2O2

CAS No. 674289-55-5 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 58 mg/mL ( (198.4 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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