2x SYBR Green qPCR Master Mix (High ROX)

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Advantages

Accurate Detection: High specific and sensitive quantitation even for low copy targets or a wide range of template samples

High Stability: Hot-start enzyme activation and convenient room-temperature reaction setup makes it suitable for automation and high throughput detection

Robustness and Versatility: High-efficiency, compatible with both fast and standard qPCR cycling methods

Cost Effective: Offered at much discounted price compared to market price

Price Comparison

Description

2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix with high concentration of ROX, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR on Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT, StepOne^TM, or StepOnePlus^TM instruments with high sensitivity, specificity and reliability.

Two premixed versions (High ROX and Low ROX) are provided for the following qPCR instruments:
High ROX	Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne^TM, StepOne Plus^TM.
Low ROX	Applied Biosystems 7500, 7500 Fast, ViiA^TM7; Stratagene MX4000^TM, MX3005P^TM, MX3000P^TM, QuantiStudio^TM 12K Flex.

Applications

Dye-based quantitative PCR detection
Nucleic acid amplification and expression profiling
Quantitative genotyping studies
Genomics-related applications

Components

Component	Cat#: B21402	Cat#: B21403
2x SYBR Green qPCR Master Mix(High ROX) *	5 mL (for 200 reactions with 50 μl/rxn for 500 reactions with 20 μl/rxn for 1000 reactions with 10 μl/rxn)	25 mL (for 1000 reactions with 50 μl/rxn for 2500 reactions with 20 μl/rxn for 5000 reactions with 10 μl/rxn)
* Contains hot-start DNA Polymerase, dNTPs, Mg²⁺, and SYBR Green I dye and ROX Dye (high concentration)

Storage (From the date of receipt)

Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.

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