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Ethidium bromide

Name: Ethidium bromide
Brand: Selleck Chemicals
SKU: S3689
Availability: InStock
Rating: 5 (7 reviews)

Cat.No.S3689

Ethidium Bromide (Homidium bromide, EtBr, EB) is an intercalating agent which resembles a DNA base pair and commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

Chemical Structure

Molecular Weight: 394.31

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Quality Control

Batch: Purity: 99.73%

99.73

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Solubility

In vitro
Batch:

DMSO : 78 mg/mL (197.81 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 7 mg/mL

Ethanol : 1 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.95mg/ml (10.02mM)	Taking the 1 mL working solution as an example, add 50 μL of 79 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.56mg/ml (1.42mM)	Taking the 1 mL working solution as an example, add 50 μL of 11.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	394.31	Formula	C₂₁H₂₀N₃.Br	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	1239-45-8	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Homidium bromide, EtBr, EB			Smiles	CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]

Mechanism of Action

In vitro

Ethidium Bromide (EtBr) is sometimes added to running buffer during the separation of DNA fragments by agarose gel electrophoresis. It is used because upon binding of the molecule to the DNA and illumination with a UV light source, the DNA banding pattern can be visualized. The mode of binding of EtBr is intercalation between the base pairs. This binding changes the charge, weight, conformation, and flexibility of the DNA molecule. The mobility of DNA was always less in the gels with EtBr.

References

[1] https://pubmed.ncbi.nlm.nih.gov/8957173/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06343311	Not yet recruiting	B-Cell Non-Hodgkin''s Lymphoma (NHL)\|Lymphoma Non-Hodgkins\|Lymphomas Non-Hodgkin''s B-Cell\|Non-Hodgkin Lymphoma\|Non-Hodgkin''s Lymphoma\|Large B-Cell Lymphoma\|Lymphoma Non-Hodgkin''s Adult\|Lymphoma\|Refractory Non-Hodgkin Lymphoma\|Relapsed Non-Hodgkin Lymphoma\|Lymphoma Non-Hodgkin\|HIV Associated Lymphoma\|CNS Lymphoma\|High-grade B-cell Lymphoma\|Refractory B-Cell Non-Hodgkin Lymphoma	Estrella Biopharma Inc.\|Eureka Therapeutics Inc.\|Estrella Immunopharma Inc.	May 15 2024	Phase 1\|Phase 2
NCT06073132	Recruiting	Generalized Epidermolysis Bullosa Simplex	TWi Biotechnology Inc.	April 2024	Phase 2\|Phase 3
NCT06378060	Recruiting	Severe Aplastic Anemia	Hematology department of the 920th hospital	March 1 2024	Phase 2
NCT05600140	Recruiting	Vision Disorders\|Vision Low	Royal Dutch Visio\|Radboud University Behavioural Science Institute Nijmegen The Netherlands	March 6 2024	Not Applicable
NCT06007235	Recruiting	Epidermolysis Bullosa Dystrophica\|Epidermolysis Bullosa Junctional	Organ Tissue Regeneration Repair and Replacement\|Euraxi Pharma\|ARANZ Medical	January 29 2024	Not Applicable
NCT05955326	Recruiting	Acute Cerebral Ischemia	Pharmazz Inc.	January 8 2024	Phase 4

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