Benzoic Acid

Catalog No.S4161 Synonyms: FEMA 2131

Benzoic Acid Chemical Structure

Molecular Weight(MW): 122.12

Benzoic acid is a colorless crystalline solid and a simple aromatic carboxylic acid, used as a food preservative.

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Biological Activity

Description Benzoic acid is a colorless crystalline solid and a simple aromatic carboxylic acid, used as a food preservative.
In vitro

Benzoic acid transports through human skin membranes with the mean maximum absorption rate of 16.54 μg/cm2/h, while the amount in the receptor fluid after 24 hours is 70.6% of the dose applied. The transport of [14C]benzoic acid is significantly inhibited carbonylcyanide p-trifluoromethoxyphenylhydrazone and nigericin, the direct driving force for benzoic acid transport is suggested to be the inwardly directed proton gradient. [1] Benzoic acid enters the diet as a natural constituent in plants, with high amounts found in fruits and berries and as a result of the widespread use of monosodium benzoate as a food preservative. Benzoic acid and para-t-butylbenzoate are most potent and acetyl CoA carboxylase (EC appeared to be the target of inhibition. Benzoic acid readily enters the cell to undergo intracellular ionization with a substantial lowering of cytosolic pH and ATP levels under acidic growth conditions. Benzoic acid is oxidized by hydroxy radicals produced by stimulated polymorphonuclear cells oxidize. [2] The photometabolism of Benzoic and 4-hydroxybenzoic acids by whole cells is inhibited by the presence of exogenous fatty acids. Benzoic acid enters the anaerobic degradation as follow: initial activation to form benzoyl-Coenzyme A, reduction of the aromatic nucleus--a reaction that has only recently been demonstrated in vitro--and the subsequent degradation of the alicyclic intermediates. [3] For Benzoic acid, there is no differences between the mean absorption through human skin and the one rat study, and skin thickness only slightly influences the absorption of Benzoic acid. [4]


Solubility (25°C)

In vitro DMSO 24 mg/mL (196.52 mM)
Ethanol 24 mg/mL (196.52 mM)
Water <1 mg/mL
In vivo

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 122.12


CAS No. 65-85-0
Storage powder
in solvent
Synonyms FEMA 2131

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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