Catalog No.S1732 Synonyms: NCI-C04933

Mitotane  Chemical Structure

Molecular Weight(MW): 320.04

Mitotane, is an antineoplastic medication used in the treatment of adrenocortical carcinoma.

Size Price Stock Quantity  
USD 97 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Purity & Quality Control

Biological Activity

Description Mitotane, is an antineoplastic medication used in the treatment of adrenocortical carcinoma.
Sterol-O-Acyl Transferase 1 [5]
In vitro

Mitotane inhibits both TSH expression and secretion, blocks TSH response to TRH, and reduces cell viability, inducing apoptosis at concentrations in the therapeutic window in the mouse TalphaT1 cell line. Mitotane does not interfere with thyroid hormone laboratory tests but directly reduces both secretory activity and cell viability on pituitary TSH-secreting mouse cells. [1] Mitotane induces adrenal cortex necrosis, mitochondrial membrane impairment, and irreversible binding to CYP proteins. Mitotane (10-40μM) inhibits basal and cAMP-induced cortisol secretion but does not cause cell death. Mitotane exhibits an inhibitory effect on the basal expression of StAR and P450scc protein. Mitotane (40 μM) significantly diminishes StAR, CYP11A1 and CYP21 mRNA expression. Mitotane (40μM) almost completely neutralizes this positive effect and returned 8-Br-cAMP-induced StAR, CYP11A1, CYP17 and CYP21 mRNA to control levels. [2] Mitotane and gemcitabine combinations show antagonistic effects and interfered with the gemcitabine-mediated inhibition of the S phase of the cell cycle in H295R cells. [3]

In vivo Mitotane (60 mg/kg) significantly decreases adrenal mitochondrial and microsomal "P-450" and microsomal protein contnet 34%, 55%, and 35% respectively in rats, within 3 hours. [4]


Cell Research:


+ Expand
  • Cell lines: NCI-H295 cells
  • Concentrations: 0-100 μM
  • Incubation Time: 24 or 72 h
  • Method:

    NCI-H295 cells (106/well) incubated with different concentrations of mitotane or diluent were collected and stained with fluorescein-isothiocyanate-labeled Annexin V for 15 minutes and for 2 minutes with propidium iodide at RT. After washing, fluorescence was measured by flow cytometry using a FACSCalibur instrument and data analyzed with Cell Quest Pro. Viability testing using WST1 reagent was performed according to manufacturer's protocol

    (Only for Reference)

Solubility (25°C)

In vitro DMSO 64 mg/mL (199.97 mM)
Ethanol 64 mg/mL warmed (199.97 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 320.04


CAS No. 53-19-0
Storage powder
in solvent
Synonyms NCI-C04933

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02057237 Completed Prostate Cancer University Health Network, Toronto September 2013 Phase 1
NCT00778817 Terminated Recurrent Adrenocortical Carcinoma|Stage III Adrenocortical Carcinoma|Stage IV Adrenocortical Carcinoma National Cancer Institute (NCI) December 2008 Phase 2
NCT00777244 Unknown status Adrenocortical Carcinoma University of Turin, Italy April 2008 Phase 3
NCT00304070 Active, not recruiting Stage I Adrenocortical Carcinoma|Stage II Adrenocortical Carcinoma|Stage III Adrenocortical Carcinoma|Stage IV Adrenocortical Carcinoma Childrens Oncology Group|National Cancer Institute (NCI) September 2006 Phase 3
NCT00094497 Completed Carcinoma, Adrenal Cortical Collaborative Group for Adrenocortical Carcinoma Treatment|German Federal Ministry of Education and Research|National Cancer Institute (NCI) June 2004 Phase 3
NCT00001339 Completed Adrenal Cortical Carcinoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) August 1993 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Related Others Products

Tags: buy Mitotane | Mitotane ic50 | Mitotane price | Mitotane cost | Mitotane solubility dmso | Mitotane purchase | Mitotane manufacturer | Mitotane research buy | Mitotane order | Mitotane mouse | Mitotane chemical structure | Mitotane mw | Mitotane molecular weight | Mitotane datasheet | Mitotane supplier | Mitotane in vitro | Mitotane cell line | Mitotane concentration | Mitotane nmr
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID