Heterostructured BiVO4/CoPi nanoarrays as high-efficiency photoanode and AuPt nanodendrites as nanozyme for sensitive sensing of miRNA 141

MicroRNA (miRNA) is a new class of tumor biomarkers in human body for early diagnosis and therapy of cancers, whose detection has scientific significance and potential applications. Herein, a sensitive heterostructured BiVO4/CoPi photoelectrochemical (PEC) biosensor was established for sensing miRNA 141 with assistance of home-synthesized AuPt nanodendrites (NDs) as nanozyme. Specifically, the BiVO4/CoPi heterostructures displayed rough worm-like internetworks, as characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). In parallel, the PEC and UV-vis diffuse reflectance spectroscopy tests confirmed their excellent optical property, combined by discussing the interfacial electron transfer mechanism. Additionally, the AuPt NDs displayed superior peroxidase-like property in the presence of H2O2 as identified by benchmarked tetramethylbenzidine (TMB) oxidation, coupled by showing remarkable catalysis for 3-amino-9-ethylcarbazole (AEC) oxidation to form biocatalytic precipitation (BCP). Integrated by a cyclic enzyme strategy, the developed PEC biosensor exhibited a wider linear range of 5 fM ∼1 pM and a lower limit of detection (LOD) as low as 0.17 fM (S/N = 3). This work provides some valuable insights for sensitive analysis of tumor-associated miRNA in clinic.

 

Comments:

This is a fascinating study! It seems like a highly technical and specialized area focusing on the development of a sensitive biosensor for detecting miRNA 141, a potential tumor biomarker. Let's break down the key elements:

1. **Objective:** The primary goal is to create a sensitive biosensor for the detection of miRNA 141, which is associated with tumors. Early detection of such biomarkers can be crucial for diagnosing cancer at an early stage and for potential therapeutic interventions.

2. **Biosensor Design:** The biosensor is based on a heterostructured BiVO4/CoPi material, which seems to have been characterized extensively using various techniques such as SEM, XRD, and XPS. These characterizations help understand the structure and properties of the materials used in the sensor.

3. **Optical Properties:** The BiVO4/CoPi heterostructures showed excellent optical properties, as confirmed by PEC and UV-vis diffuse reflectance spectroscopy tests. Understanding these properties is crucial for the sensor's functioning.

4. **Nanozyme Use:** The study employed home-synthesized AuPt nanodendrites (NDs) as a nanozyme. Nanozymes mimic the function of natural enzymes and are utilized here for their peroxidase-like properties, essential for the detection process.

5. **Catalytic Properties:** The AuPt NDs exhibited excellent catalytic properties, as indicated by their peroxidase-like behavior in the presence of H2O2. This catalytic activity was demonstrated using benchmarked tetramethylbenzidine (TMB) and 3-amino-9-ethylcarbazole (AEC) oxidation reactions.

6. **Performance of the Biosensor:** The developed PEC biosensor showed promising performance characteristics. It exhibited a wide linear detection range from 5 fM to 1 pM and a low limit of detection (LOD) as low as 0.17 fM, indicating high sensitivity for miRNA 141 detection.

7. **Significance:** The study concludes by highlighting the importance of this work for sensitive analysis of tumor-associated miRNA in clinical settings, potentially offering a new approach for early cancer diagnosis and therapy.

Overall, this work seems to have made significant strides in developing a highly sensitive biosensor for detecting a specific tumor-associated miRNA. It integrates various advanced materials, characterization techniques, and catalytic properties to achieve its objective, potentially impacting early cancer diagnosis and treatment strategies in the future.

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