ATPase Inhibitors

Cat.No.	Product Name	Information	Product Use Citations
S7046	Brefeldin A (BFA chemical)	Brefeldin A (BFA) is a lactone antibiotic and ATPase inhibitor for protein transport with IC50 of 0.2 μM in HCT 116 cells, induces cancer cell differentiation and apoptosis. It could also improve the HDR(homology-directed repair) efficiency and be an enhancer of CRISPR-mediated HDR. Brefeldin A is also an inhibitor of autophagy and mitophagy.	Protein Cell, 2025, pwaf020 Cancer Commun (Lond), 2025, 10.1002/cac2.70036 J Integr Plant Biol, 2025, 67(8):2229-2244
S7099	(-)-Blebbistatin	(-)-Blebbistatin ((S)-(-)-Blebbistatin) is a cell-permeable inhibitor for non muscle myosin II ATPase with IC50 of ~2 μM in cell-free assays, does not inhibit myosin light chain kinase, inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly.	Nat Commun, 2025, 16(1):9578 Nat Commun, 2025, 16(1):1012 Cell Biosci, 2025, 15(1):146
S8101	CB-5083	CB-5083 is a potent, selective, and orally bioavailable p97 AAA ATPase inhibitor with IC50 of 11 nM. Phase 1.	Nat Commun, 2025, 16(1):3546 EMBO J, 2025, 10.1038/s44318-025-00517-x Proc Natl Acad Sci U S A, 2025, 122(27):e2426218122
S7895	Thapsigargin	Thapsigargin is a potent, non-competitive inhibitor of the sarco/endoplasmic reticulum Ca²⁺ ATPase (SERCA) with IC50 of 0.353 nM or 0.448 nM for the carbachol-evoked [Ca2+]i-transients with or without a KCl-prestimulation. Thapsigargin induces cell apoptosis. Thapsigargin is extracted from a plant, Thapsia garganica.	EMBO J, 2025, 44(4):1107-1130 Proc Natl Acad Sci U S A, 2025, 122(20):e2502652122 Cell Death Dis, 2025, 16(1):289
S4290	Digoxin	Digoxin is a classical Na,K-ATPase inhibitor, with selectivity for the α2β3 isoform over the common α1β1 isoform, used in the treatment of atrial fibrillation and heart failure.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.	J Exp Clin Cancer Res, 2025, 44(1):294 Pharmaceuticals (Basel), 2025, 18(2)181 Drug Metab Dispos, 2025, 53(4):100049
S7266	Golgicide A	Golgicide A is a potent and rapidly reversible GBF1 inhibitor.	Stem Cell Research & Therapy, March 7, 2025, 121 Stem Cell Research & Therapy, March 07, 2025, 121 Journal of Biological Chemistry, February 10, 2017, 2315-2327
S2000	Sodium orthovanadate	Sodium orthovanadate is an alkaline phosphatase and (Na,K)-ATPase inhibitor with IC50 of 10 μM.	PLoS Pathogens, July 31, 2020, e1008701 Cancer Gene Therapy, February 6, 2025, 286-296 Cancer Gene Ther, 2025, 10.1038/s41417-025-00874-z
S7821	Bufalin	Bufalin (BF) is a potent inhibitor of steroid receptor coactivator SRC-3, SRC-1 and Na+/K+-ATPase. This compound binds to the subunit α1, α2 and α3 of Na+/K+-ATPase with Kd of 42.5 nM, 45 nM and 40 nM, respectively. It is a major immunoreactive component isolated from the Chinese medicine Chan Su with anti-cancer activity.	Eur J Med Chem, 2026, 301:118197 J Control Release, 2025, 383:113814 Dissertation, LMU München: Medizinische Fakultät, 2025, nan
S2233	Esomeprazole sodium	Esomeprazole Sodium((S)-Omeprazole sodium, (-)-Omeprazole sodium) is a sodium salt of esomeprazole that is a potent proton pump inhibitor with an IC50 of 0.076 mg/kg.	iScience, 2024, 27(10):110862 J Pers Med, 2022, 12(2)258 Hum Cell, 2022, 10.1007/s13577-022-00671-y
S3019	Ciclopirox ethanolamine	Ciclopirox ethanolamine (Ciclopirox olamine, HOE 296,Ciclopiroxolamine) is a broad-spectrum antifungal agent working as an iron chelator.	Analytical Cellular Pathology, May 19, 2022, 4213401 Proceedings of the National Academy of Sciences, December 6, 2022, e2208458119 Communications Biology, April 13, 2023, 402
S2528	Ciclopirox	Ciclopirox (HOE 296b, Penlac) is a broad-spectrum antifungal agent working as an iron chelator. This compound is a broad-spectrum antifungal agent working as an iron chelator. This chemical is a broad-spectrum antifungal agent working as an iron chelator.	Journal of Translational Medicine, August 13, 2021, 347 Cell Death Dis, 2021, 12(12):1141 J Transl Med, 2021, 19(1):347
S5608	Pantoprazole sodium hydrate	Pantoprazole (BY10232, SKF96022) sodium hydrate (Protonix) is a potent inhibitor of H+/K(+)-ATPase with IC50 of 6.8 μM.	Molecular Therapy, 2025, 336-355 Eur J Drug Metab Pharmacokinet, 2022, 10.1007/s13318-022-00780-x Journal of Virology, 2021, e00190-21
S5623	Bedaquiline	Bedaquiline (TMC-207, R207910), a diarylquinoline, binds to subunit c of mycobacterial ATP synthase and inhibits its activity. It is highly selective for the mycobacterial ATP synthase enzyme compared to the homologous eukaryotic enzyme (Selectivity Index >20 000).	Sci Rep, 2023, 13(1):3825 Microbiol Spectr, 2023, e0046523. Adv Healthc Mater, 2022, e2102117
S3924	Ginsenoside Rb1	Ginsenoside Rb1 (Gypenoside Ⅲ) is a protopanaxadiol that has diverse in vitro and in vivo effects, including neuroprotective, anti-inflammatory, and anti-obesity actions. This compound, a main constituent of the root of Panax ginseng, inhibits Na+, K+-ATPase activity with IC50 of 6.3±1.0 μM. It also inhibits IRAK-1 activation and phosphorylation of NF-κB p65. This chemical reduces the expressions of TLR3, TLR4 and TRAF-6, and down-regulates the levels of TNF-α, IFN-β and iNOS.	J Biol Chem, 2024, 300(8):107542 J Ethnopharmacol, 2021, S0378-8741(21)00169-0 J Biol Chem, 2021, S0021-9258(21)00790-0
S7460	BTB06584	BTB06584 is an IF1-dependent, selective inhibitor of the mitochondrial F1 Fo-ATPase.	Oncotarget, June 27, 2017, 53602-53612 International Journal of Molecular Sciences, August 5, 2023, 12460 Oncotarget, August 08 2017, 53602-53612
E1251	Oligomycin	Oligomycin is a macrolide antibiotic produced by Streptomyces. It is a mixture of three isomers, namely Oligomycin A, B and C. This compound is an inhibitor of mitochondrial F0 subunit H+-ATP synthase, which can disrupt oxidative phosphorylation and the electron transport chain. It can inhibit TNF-induced apoptosis and can be used as an antifungal antibiotic.	J Allergy Clin Immunol, 2025, S0091-6749(25)00948-0 J Cell Biol, 2025, 224(8)e202411078 Front Immunol, 2025, 16:1571221
S5454	Saikosaponin D	Saikosaponin D, a calcium mobilizing agent (SERCA inhibitor), is a type of Saponin derivative, which is a component extracted from Bupleurum falactum. This compound has anti-cancer activities.	Scientific Reports, 2026, Vol 16, Article number: 1 Theranostics, 2025, 8935-8951 Theranostics, 2025, 15(17):8935-8951
S4166	Chlorpropamide	Chlorpropamide inhibits Na(+),K(+)-ATPase and stimulates a high affinity cyclic AMP-phosphodiesterase of isolated liver plasma membrane. Chlorpropamide is a sulfonylurea class drug for type 2 diabetes mellitus.	Cancer Biology & Medicine, January 15, 2022, 19(1):74-89 Nat Commun, 2017, 8(1):573
S3577	Rhodamine 123	Rhodamine 123 (RH-123, R-22420) is a fluorescent cationic dye used to label mitochondria in living cells. This compound inhibits ADP-stimulated respiration of mitochondria with Ki = 12 μM and ATPase activity of inverted inner membrane vesicles with Ki of 126 μM and partially purified F1-ATPase with Ki of 177 μM.	Am J Transl Res, 2023, 15(6):4065-4078
E0054	Tegoprazan	Tegoprazan (CJ-12420, IN-A001, K-CAB, LXI-15028, RQ-00000004) is a novel potent and highly selective inhibitor of gastric H+/K+-ATPase with IC50s ranging from 0.29 μM to 0.52 μM for porcine, canine, and human H+/K+-ATPases in vitro, respectively.	Scientific Reports, November 8, 2024, 27173 Sci Rep, 2024, 14(1):27173
E1311	Paxilline	Paxilline, an indole alkaloid mycotoxin derived from Penicillium paxilli, potently inhibits the BK channel by exerting its effects through direct channel inhibition. Additionally, this compound also functions as an inhibitor of sarco/endoplasmic reticulum Ca2+-stimulated ATPase (SERCA), with IC50 values ranging from 5 μM-50 μM, depending on the SERCA isoform. It also possesses significant anticonvulsant and neuroprotective effects.
E0244	Acevaltrate	Acevaltrate, an active component derived from the herbal plant Valeriana jatamansi Jones, is strikingly potent to induce GBM cell apoptosis. This compound inhibits the Na+/K+-ATPase activity in the rat kidney and brain hemispheres with IC50 of 22.8 μM and 42.3 μM, respectively.
S0161	ML367	ML367 is a potent, micromolar inhibitor of ATPase family AAA domain-containing protein 5 (ATAD5) stabilization. This compound blocks DNA repair pathways and general DNA damage responses including RPA32-phosphorylation and CHK1-phosphorylation in response to UV irradiation.
E0680	Deslanoside	Deslanoside(Desacetyllanatoside C) is a cardenolide glycoside, has the effects of anti-arrhythmia and cardiotonic, also is used as an EC 3.6.3.9 (Na⁺/K⁺-transporting ATPase) inhibitor.
S0166	BRITE338733	BRITE-338733 is a potent RecA (a DNA-dependent ATPase) inhibitor with IC50 of 4.7 µM.
S6918	Oleandrin (PBI-05204)	Oleandrin (PBI-05204, Foliandrin, Neriolin, Neriostene, Folinerin, Corrigen, Neriol) is an inhibitor of Na+/K+-ATPase with IC50 of 0.62 μM. This compound induces apoptosis and reduces migration of human glioma cells in vitro.
E1983^New	BAY-850	BAY-850 is a potent and isoform-selective inhibitor of the ATPase family AAA domain-containing protein 2 (ATAD2) bromodomain, with an IC50 of 166 nM in TR-FRET assays. It induces ATAD2 bromodomain dimerization and effectively blocks its interaction with acetylated histones in vitro.
S0219	ML241 hydrochloride	ML241 hydrochloride is a potent and selective inhibitor of p97 with IC50 of 0.11 μM and 3.5 μM for p97 ATPase and Ub^G76V–GFP, respectively.
S0856	KM91104	KM 91104 is a cell-permeable inhibitor of V-ATPase that specifically targets the interaction between V-ATPase subunit a3 and subunit B2.
S1413	Bafilomycin A1 (Baf-A1)	Bafilomycin A1 (Baf-A1) is a vacuolar H⁺-ATPase inhibitor with IC50 of 0.44 nM, and it is found to inhibit autophagy while inducing apoptosis.	Cell & Bioscience, July 05, 2025, 96 Journal of Cellular and Molecular Medicine, February 28, 2021, 3348-3360 Cells, August 09, 2025, 1231
S1478	Oligomycin A (MCH 32)	Oligomycin A (MCH 32), a dominant analog of the isomers, is an inhibitor of mitochondrial F1FO ATP synthase which inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. It inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. It also induces apoptosis in a variety of cell types.	Nat Commun, 2025, 16(1):212 Int J Biol Sci, 2025, 21(1):233-250 Cell Rep, 2025, 44(7):115973
S8276	FCCP	FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.	Nature, 2025, 10.1038/s41586-024-08395-9 Nat Commun, 2025, 16(1):3012 Cell Death Dis, 2025, 16(1):163
S1389	Omeprazole	Omeprazole is a proton pump inhibitor that blocks H(+)-K(+)-ATPase, used to treat dyspepsia, peptic ulcer disease, gastroesophageal reflux disease, laryngopharyngeal reflux, and Zollinger–Ellison syndrome.	Cells, 2025 Sep 24, 1489 Gut, 2025, gutjnl-2024-334561 Gut, 2025, nan
S2343	Phlorizin	Phlorizin (Phloridzin) is a dihydrochalcone found in the bark of pear (Pyrus communis), apple, cherry and other fruit trees. This compound is a non-selective SGLT inhibitor with Ki of 300 nM and 39 nM for hSGLT1 and hSGLT2, respectively. It is also a Na+/K+-ATPase inhibitor.	Journal of Ginseng Research, 2022, 700-709 Mol Ther Methods Clin Dev, 2022, 24:11-19 J Ginseng Res, 2022, 46(5):700-709
S3666	Ilaprazole	Ilaprazole (IY-81149) is a new proton pump inhibitor (PPI) used in the treatment of dyspepsia, peptic ulcer disease (PUD), gastroesophageal reflux disease (GORD/GERD) and duodenal ulcer. It inhibits H+/K+-ATPase with an IC50 of 6.0 μM.	bioRxiv, May 19, 2025, 2025.05.19.654850 Journal of Virology, May 10, 2021, 95(11):e00190-21 Journal of Virology, June 10, 2021, 95(13):e02466-20
E1166	Tetrathiomolybdate (TM)	Tetrathiomolybdate (TM) is used in the clinic for the treatment of Wilson’s disease by inducing dimerization of the metal-binding domain of the cellular copper efflux protein ATP7B (WLN4) through a unique sulfur-bridged Mo2S6O2 cluster.	Phytomedicine, 2025, 143:156883 The FASEB Journal, 2025, e71250 J Exp Clin Cancer Res, 2024, 43(1):68
S2105	Pantoprazole	Pantoprazole (SKF96022, BY-1023) is a proton pump inhibitor drug that inhibits gastric acid secretion. It works on gastric parietal cells to irreversibly inhibit (H+/K+)-ATPase function and suppress the production of gastric acid.	Journal of Virology, May 10, 2021, e00190-21 Med Oncol, 2024, 41(8):188 Int J Mol Sci, 2022, 23-158572
S1354	Lansoprazole	Lansoprazole (A-65006, AG-1749) is a proton-pump inhibitor (PPI) that binds covalently to parietal cell H(+),K(+)-ATPase. This compound prevents the stomach from producing gastric acid.	Journal of Virology, May 10, 2021, e00190-21 bioRxiv, 2023, 548707 Frontiers in Cell and Developmental Biology, 2021, 655559
S4099	Dexlansoprazole	Dexlansoprazole (T 168390, TAK 390,(R)-Lansoprazole), the dextrorotatory enantiomer of lansoprazole, is a proton pump inhibitor (PPI) formulated to have dual delayed-release properties. This compound selectively suppresses gastric acid secretion by direct inhibition of the H(+),K(+)-ATPase proton pump in the gastric parietal cell.	Food Chem Toxicol, 2025, 197:115262 J Immunother Cancer, 2024, 12(11)e009805 Journal of Virology, 2021, e00190-21
S3628	BHQ	BHQ (2,5-di-t-butyl-1,4-benzohydroquinone) is a potent and selective inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA).	European Journal of Pharmacology, 2024, 176585 Diabetes, 2022, 1660-1678 Diabetes, 2022, 71(8):1660-1678
S4538	Pantoprazole sodium	Pantoprazole (SKF96022, BY-1023) is a proton pump inhibitor drug that inhibits gastric acid secretion. It works on gastric parietal cells to irreversibly inhibit (H+/K+)-ATPase function and suppress the production of gastric acid.	Journal of Virology, May 10, 2021, e00190-21 Eur J Drug Metab Pharmacokinet, 2022, 10.1007/s13318-022-00780-x J Virol, 2021, JVI.00190-21
S8828	Gboxin	Gboxin is an inhibitor of oxidative phosphorylation in cancer cells. This compound inhibits the activity of F0F1 ATP synthase. It specifically inhibits the growth of primary mouse and human glioblastoma cells but not that of mouse embryonic fibroblasts or neonatal astrocytes.	Cell Rep, 2025, 44(7):115901 Cell Rep, 2024, 43(10):114775 Mol Oncol, 2023, 17(9):1821-1843
S4212	Tenatoprazole	Tenatoprazole (TU-199,STU-Na) is a prodrug of the proton pump inhibitor (PPI) class, which inhibits proton transport with IC50 of 3.2 μM. This compound labels only the gastric H(+),K(+)-ATPase alpha-subunit, approximately 2.6 nM/mg of this chemical is bound to the H(+),K(+)-ATPase.	Advanced Science, 2024, e2308312 Viruses, 2024, 16(10)1566 J Virol, 2021, JVI.00190-21
S1743	Esomeprazole magnesium	Esomeprazole Magnesium(NEXIUM) is a proton pump inhibitor to reduce gastric acid secretion through inhibition of H(+)/K(+)-ATPase in gastric parietal cells.	Scientific Reports, August 17, 2017, 8491 Scientific Reports, 2017, 8491
S5502	Ilaprazole sodium	Ilaprazole (IY-81149) sodium is the sodium salt form of ilaprazole, which is a proton pump inhibitor. Ilaprazole sodium irreversibly inhibits H(+)/K(+)-ATPase in a dose-dependent manner with IC50 of 6 μM in rabbit parietal cell preparation.	J Virol, 2021, JVI.00190-21
S5058	Revaprazan Hydrochloride	Revaprazan Hydrochloride (YH1885) is a new reversible proton pump inhibitor with long-lasting acid-suppressive effects. This compound reversibly inhibits H(+)/K(+)-ATPase via binding to the K+-binding site of the pump.	ACS Infect Dis, 2021, 10.1021/acsinfecdis.0c00754
S2222	PF-3716556	PF 3716556 is a potent and selective P-CAB (potassium-competitive acid blocker), with pIC50 of 6.026 and 7.095 for the inhibition of porcine H⁺,K⁺-ATPase activity in ion-leaky and ion-tight assay, respectively, inhibits gastric acid secretion, displays no activity at Na⁺,K⁺-ATPase, used for the treatment of gastroesophageal reflux disease.
S3143	Pyruvic acid	Pyruvic acid (Acetylformic acid) is an important organic chemical intermediate that plays a role in cardiomyocyte pathophysiology and therapy. This compound markedly increases the level of lactate dehydrogenase (LDH) and creatine kinase (CK) and reduces the level of Ca²⁺Mg²⁺-ATPase and Na⁺K⁺-ATPase.