Home Transmembrane Transporters ATPase inhibitor - DNA/RNA Synthesis inhibitor ML367 For research use only.

ML367

ML367 is a potent, micromolar inhibitor of ATPase family AAA domain-containing protein 5 (ATAD5) stabilization. ML367 blocks DNA repair pathways and general DNA damage responses including RPA32-phosphorylation and CHK1-phosphorylation in response to UV irradiation.

ML367 Chemical Structure

ML367 Chemical Structure

CAS: 381168-77-0

Purity & Quality Control

Batch: S016101 DMSO] 67 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.96%
99.96

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Biological Activity

Description ML367 is a potent, micromolar inhibitor of ATPase family AAA domain-containing protein 5 (ATAD5) stabilization. ML367 blocks DNA repair pathways and general DNA damage responses including RPA32-phosphorylation and CHK1-phosphorylation in response to UV irradiation.
Targets
ATAD5 [1]
In vitro
In vitro

ML367 is a probe molecule found to block general DNA damage responses including RPA32-phosphorylation and CHK1-phosphorylation in response to UV irradiation. In this regard, the probe molecule could block DNA repair pathways that function upstream of ATAD5. Additionally, ML367 sensitizes cells possessing a knock-out mutation of the PARP1 gene and as a result may serve as a sensitizer to kill cancer cells defective in the poly (ADP-ribose) polymerase 1 (PARP1)-dependent DNA repair pathway.[1]
Cell Research Cell lines HEK293T cells
Concentrations 5, 10, 20 and 40 μM
Incubation Time 16 h
Method

HEK293T cells were transiently transfected with FLAG-tagged ATAD5 and treated with the indicated amount of ML367 for 16 hours in the presence or absence of 20 μM 5-FUrd. ATAD5 protein levels were visualized by western blotting using an antibody against FLAG, and quantified using ImageJ.

Chemical Information & Solubility

Molecular Weight 334.32 Formula

C19H12F2N4
CAS No. 381168-77-0 SDF --
Smiles C1=CC=C2C(=C1)C(=NC(=N2)C3=CC=NC=C3)NC4=CC(=C(C=C4)F)F
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 67 mg/mL ( (200.4 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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