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Bedaquiline ATPase inhibitor

Name: Bedaquiline
Brand: Selleck Chemicals
SKU: S5623
Availability: InStock
Rating: 5 (5 reviews)

Cat.No.S5623

Bedaquiline (TMC-207, R207910), a diarylquinoline, binds to subunit c of mycobacterial ATP synthase and inhibits its activity. It is highly selective for the mycobacterial ATP synthase enzyme compared to the homologous eukaryotic enzyme (Selectivity Index >20 000).

Chemical Structure

Molecular Weight: 555.50

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Quality Control

Batch: Purity: 99.99%

99.99

Related Targets	CFTR CRM1 CD markers AChR Calcium Channel Sodium Channel Potassium Channel GABA Receptor TRP Channel GluR
Other ATPase Inhibitors	(-)-Blebbistatin Thapsigargin Brefeldin A (BFA chemical) CB-5083 Golgicide A Sodium orthovanadate Bufalin CDN1163 Ginsenoside Rb1 Oligomycin

Solubility

In vitro
Batch:

DMSO : 33 mg/mL (59.4 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.670mg/ml (3.01mM)	Taking the 1 mL working solution as an example, add 50 μL of 33.3 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	555.50	Formula	C₃₂H₃₁BrN₂O₂	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	843663-66-1	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	TMC-207, R207910			SMILES	CN(C)CCC(C1=CC=CC2=CC=CC=C21)(C(C3=CC=CC=C3)C4=C(N=C5C=CC(=CC5=C4)Br)OC)O

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	mycobacterial ATP synthase
In vitro	Bedaquiline targets against Mycobacterial ATP synthase complex. This compound has anti-cancer activity, directed against Cancer Stem-like Cells (CSCs). Its treatment of MCF7 breast cancer cells inhibits mitochondrial oxygen-consumption, as well as glycolysis, but induces oxidative stress. It reduces mitochondrial membrane potential, with a significant rise in ROS levels. It specifically inhibits mitochondrial respiration in cancer cells, while enhancing mitochondrial function in normal cells. This chemical dramatically inhibits oxygen consumption and ATP production in the low micromolar range. In addition, its treatment also reduced aerobic glycolysis.
In vivo	Bedaquiline follows triphasic elimination and characterized by an outstandingly long terminal half-life, around 173 h in humans.
References	[1] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930122 [2] https://pubmed.ncbi.nlm.nih.gov/27344270/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-06-30)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07675954	NOT_YET_RECRUITING	Rifampicin-Resistant Tuberculosis	Seoul National University Hospital	2026-10-01	PHASE3
NCT06441006	RECRUITING	Tuberculosis, Pulmonary; Tuberculosis, Multidrug-Resistant; Tuberculosis, MDR	University of California, San Francisco	2025-10-03	PHASE2; PHASE3
NCT07485010	NOT_YET_RECRUITING	Mycobacterium Abscessus Pulmonary Disease; Mycobacterium Abscessus Infection; Non-Tuberculous Mycobacterial (NTM) Infections; Non-Tuberculous Mycobacteria Pulmonary Disease	The University of Queensland	2027-04	PHASE2
NCT07486024	NOT_YET_RECRUITING	MDR-TB; Tuberculosis Multi Drug Resistant Active; Antibiotic Resistance; Mycobacterium Tuberculosis	Assistance Publique - Hôpitaux de Paris	2026-04	PHASE3
NCT07076225	NOT_YET_RECRUITING	Pulmonary Tuberculosis	Shenzhen Third People's Hospital	2025-07-25	PHASE3
NCT04310930	RECRUITING	Pulmonary Disease Due to Mycobacteria (Diagnosis)	The University of Queensland	2020-03-02	PHASE2; PHASE3

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